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ҽҩȫ > ҩ > ҩ > B > ˽

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2017-11-02 14:22 Ķ:

йҩ䡷˽

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ƴB Jio Huí Xin

ӢFRUCTUS ANISI STELLATI

ԴƷΪľֲ˽Illicium verum Hook. f. ʵʵ̱ʱժ÷ˮֱ̺Ӹ

״ƷΪۺϹ8uȋɣ״ϡuȋ12cm0.30.5cm0.61cmɫвƣ˳״ϲ࿪ѣڱ浭ɫƽйӲࡣ34cmڹʵ룬䡣ÿuȋ1ԲΣԼ6mmɫɫꣻɫԡ㣬ζ

𡿣1 ƷĩɫڹƤդ״ϸΣ200546μm Ժƿ׿ʮ״״ƤʯϸɫΣڼ񣬲״ǻ֦״ںغɫ۳Σڲ񡣹Ƥʯϸ೤ΡԲλ֦״ںάɢڻֱ2960μm ľƿסйƤϸɫɢϸϸΣ֬͵κͺ

2 ȡƷַ1gʯѣ6090棩-ѣ1:1 Һ15mlҡ15ӣ˹ҺˮԡϻӸɣˮҴ2ml ʹܽ⣬ΪƷҺȡƷҺ2μlȼ׻άΪϼĹ轺DZϣӸɣٵӼ䱽ҺԼ2μlԷۺɫϺɫԲ

3 ȡ[]2 µĹƷҺ10μl,10mlƿУˮҴ̶ȣҡȣշֹȷ¼ Aⶨ259nm ա

4 ȡ˽ҩ1g[]2 µĹƷҺƱƳɶҩҺȡȩƷ20μl,ˮҴ2ml ʹܽ⣬ΪƷҺձɫ׷¼ B飬ȡ[]2 µĹƷҺҺ510μlֱͬһȼ׻άΪϼĹ轺DZϣʯѣ3060棩-ͪ-19:1:1ΪչչȡɣԼ䱽ҺƷɫУҩɫӦλϣͬɫİߵ㣻ƷɫӦλϣͬijɫȺɫߵ㡣

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Ρɢֹʹں޸ʹʹθŻ£丹ʹ

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ȫвҩࡷ˽

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ƴB Jio Huí Xin

㡢ϡ˽

Դľư˽ֲ˽Illicium verum Hook. F.ʵҩ^գɹɱá

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ѧɷ֡ʵӷ49һԼΪ5Ƥн϶ࣩ֬Լ22Уʡ֬ȡӷҪɷΪѣanetholeԼ8090ʱΪdϩlˮϩαƷѡ׻ӣmethyl chavicolҶӷͣкѡȩȡ

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ƴB Jio Huí Xin

㣨ηҪ㣨ˡ㡢˽飨Ŀ˽㡢˽Ǵ桷˽ǣԭԭҩϻࡷ˽ǣҩѧ

Ʒ㾫Ҫ

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ֲɵʪɽءҰࡣֲ㶫ϡݡ̨ȵء

״ʵ8613uȋɾۺϹ״Уһ״ĹݼԹСͧΣ520ף510ףԼ5ף˶ۼƽֱԵѡƤɫƣڱ浭ɫйں1ӱΣ7ף4ף2ףƤɫɫһС꣬ףһкϵ㣬ϵ֮еɫϸּƤʴ࣬ںɫʣʡζ΢Ըɫ졢ͶࡢŨΪѡ

㶫ϵȵء

ֲͬçĹʵ״˽dzƣ׻çݹʵжáҪΪçݹʵСuȋһ㳤Լ710ף˳֮״ഹֱ䡣ζζࡣ

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㡢㡢㡢˽顢˽㡢˽Ǵ˽ǡԭ顢ϡ˽

ԡƷ㾫Ҫ

ԴҩĻԴΪ˽ǿֲ˽Ĺʵ

ֲﶯIllicium verum Hook.f.

պʹأ810ʢڣ5070ꡣ4¼ʵʱʰȡɹɡ10-11²գɺ÷ˮУԼ510min̳ɹɻɡ

ԭ̬ľ10-20mƤɫɫвơ֦ܼˮչҶ3-6֦Ҷ׳Լ1cmҶƬʣԲλԲ״Σ6-12cm2-4cmȶ˽򼱼⣬ШΣȫԵɫй͵㣬dzɫëԣҶҸƬ7-12֣״Уַۺɫ11-19ų1-2֣Ƥ8-9ۺϹ8ܳǣܳͻ״гɰ˽Σֱ3.5-4cmɫľʣܳǣܳͻȶ˶ۼۣʱظ߿ѡ1Σɫڴ.^괺

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Դֲֲڸ̨塢㶫ݡϵȵء

ࡿѧ ϲůʪ򣬲ڱγ25°ϣ꽵ˮ1000mmϣʪ80ϡ

¶ƽ1923棬ƽ10ϡϲ񣬳ϲ⡣ǿ͸ɺǿ硣ɡֳʺḻˮõƫԵɰΪˡ

༼ ӷֳѡʮֲꡢʵ࣬޲溦910²չƤɫƺɫijʵ沥ʪɳ212²֡о1520cm4cm34cm1òݻҰϸϸǣԼ3cmõݻéݸǡǰҪˮٽѿȥ֦11ٲȥ紲ҪݣʩʣԵΪסطΪּ2ѿδȶǰСʵ2磬һΪ5mңÿ1hm2 390ꣻҶ3磬Ϊ133mңÿ1hm2 5 625ꡣ

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״״ ۺϹԼ8ϡ5-13ܳǣӢ۳ɣֹȴ󣬷״ϣܳǣܳͻ1-2cm0.5-1cmغɫɫвƣ˶ۻۼ⣬ƤϺϲ࿪ѳСͧΣڱ浭ɫйÿܳǣܳͻ1ԲΣɫɫй󡣹3-4cm䡣㣬ζ

Ըɫ졢ԴŨΪѡ

΢ ʵ棺ƤΪ1бƤϸⱻСͻĽʲ㡣йƤΪϸΪϸɢڵϸάڸߴкϸڹƤΪ1״ϸڸ߲ΪʯϸʯϸӸ𽥼ӳ״ϸνӡƤƤϸΪ1нܵijʯϸڳUΪӪ㱡ϸϸ֬ͼ

ĩɫٹƤƤϸΣں񣬽ܡײʽԲλԲΣֱ40-45μmϸ4-8ڸʯϸ೤λΣ260μmп׹ƿסϸ飬ԲΣֱ150-180μmڹƤ״ϸֱ50-80μm500μmڽϱľߵбƿ׻ʮƿ׶ԡƤƤʯϸɫΣ50-80μm180μmάϴֳ40-90μm1000μmƿԣľ

1ȡƤַ05g,Ҵ5ml½2min䣬˹Һˮ25mlŻǣҺ©Уʯ10mlҡãȡʯҺɣӴܽ⣬ȻҺ2ΣҡعܱڻᣬҺ㽻紦Գ־õɫѣ2ɫ ȡƷַ10gȡӷͣ1mlȷУáȡȷҺΪҺֱͬһ轺Gϣʯ-991չ5ȩԼɫƷɫɫӦλһӣɫߵ㡣

Ʒʱ־ л񹲺͹ҩ䡷1995涨ƷӷͲ40mlg

ѧɷ֡ʵͪ໯УƤ-3O-߰quercetin-3O-rhamnosideƤ-3O-߰quercetin-3O-β-glucosideƤ-3O-߰quercetin-3O-galactosideƤ-3O-ľ߰quercetin-3O-xylosideƤأquercetinɽηӣkaempferolɽη-3O-߰kaempferol-3-O-glucosideɽη-3O-߰Kaempferol-3Obalactosideɽη-3-ܿ߰kaempferol-3-rutinoside[1]л໯34-5-ᣨcaffeoylquinic acid345-κᣨferuloylauinic acid4-β-D--[4-β-D-glucopyra-nosyloxybenzoic acid[2]ǻƤᣨhydroxycinnamic acidǻᣨhydroxybenzoic acid[3]ȡֺӷͣɷǷʽԣanetholeжԱϩϩѣfoeniculinα-β-ϩpineneϩcamphene¹ϩmyrceneα-ˮϩaphellandreneα-ϩαlimonene3-ϩ3carene֦Ҷأcineole410-ϩ410thujene],α-ϩα-terpinenelinaloolα-ʹα-terpineol4-ʹ4terPineolԣestragole˳ʽԣȩanisaldehydeα-ϩαbergamotene˳ʽ-β-ϻϩZ-β-farneseneʽϩ caryophylleneԱȩtereph-thaldehydeβ-ûҩϩβbisaboleneα-ϩα-humulene3-methyl 3methoxy enzoate,β-ϩβ-se-linene,α-ţͣϩα-copaene,Լ-2-ͪp-methoxyphenylpropan-2oneδ-γ-ϩcadinene,β-ľϩβ-guaieneȻ崼nerolidol㴼elemol׻춡ͷӣmethylisoeugenolβ-ϩβ-maalieneܲδcarotolľcedrol[4]ԼƤȩpmethoxycinna-maldehyde[5]

ҩá1.־ Ʒˮͽ˸˾ݲݸ˾־áҴȡԽɫ׺˾ݲݸ˾һ˺˾˺˾˾󳦸˾²нǿá

ȡԸϸ(ɫ׺˾)֮ùؼ20λ/mlƣԸϸ(ݲݸ˾󳦸˾һ˺˺˾)֮־ù50λ/mlƣ֮־ô1%ıἰˮᡣ

2.̼ ӷеѾд̼ãܴٽθ䶯ɻ⸹ʹԺϸд̼öٽڡ̵

3.ϸ Ʒȡ׻ӣúͺiml00mg/ֻҩ24СʱϸΪҩǰ150%(P0.05Ȯim300mg/ֻҩ24Сʱҩϸӣͣҩ2СʱϸΪҩǰ157%ϸΪҩǰ188%кϸʻԾ״̬Ȯ1po200mg/ֻ24Сʱϸߣ48СʱΪҩǰ161%P0.05ֱӹ볦6-24Сʱ԰ϸΪҩǰ166-181%(P0.01)Ȯûµİϸ֢ͬʱü׻ʹȮȫϸ½ָ졣ԻƺͷƲ˵İϸ֢нϺЧ

4.ߴƼػ ƷԾдƼػԣΪ50СM.U./ml100λ(R.U./ml)

ԡ1.ﶫز(ͳ)͹Ʒ(ʳö)˽ȡĻӷͣÿ1kg259igС󣬹۲7dδ

2.Сpo4g/kgip1.5g/kgԵ˳ʽ칹ip0.07g/kgС0.095g/kgʽip2.67g/kgС1.41g/kgʽ칹壬ipΪ900mg/kg˳ʽ칹壬ipΪ93mg/kg˽ɷְ(anethole)θСLD50Ϊ4.0g/kgǻעΪ1.5g/kg˳ʽ칹帹ǻעԴLD50Ϊ0.07mg/kgСΪ0.095mg/kgʽ칹帹ǻעԴLD50Ϊ2.67mg/kgСLD50Ϊ1.41mg/kg

3.׻ӵLD50Сpo4000mg/kg

4.ͻ飺ֲоֲ԰()ṩĸɹˮûӷͣTA98ͻ;꼰TA100û;꣬涨ŴԡԷرԼԶջԡ(1985)˺ɳϾձ鷽ֽƬԺ뷨ÿ0.2220500ȼΧ(ug/)ÿһ2Σÿ2-3ƽڼ벻S9·ֱвԡ˽δ⵽ձԣS92-(2һAAF)Rt/Rc≥2ձԡȡʳܰ߷ػ۰˽(δѧ)Ƥȣȡ0.5gֱӶ1ml24hմнҺĿֱԷͻ飬ͽжϾȫAmes׼У벻Sһ9뷨˽ͻTA98ԷӦTA100ý(±)ƤΪԡʵʾ3ȻζƷİȫֵýһо

1.ȡƤַ0.5gҴ5ml½2min䡢ˡҺмˮ25mlɫǣҺ©Уʯ10mlҡãȡʯѣɡӴ2mlʹܽ⣬ȻҺ2Σҡȡ˴Һعܱڻʢ(Լ1-2ml)ԹУҺԳһԡ־õɫ()2.ȡƷַ10gȡӷͣ1mlȷУãѺȩȷҺΪաֱ轺Gϣһʯ-(991)չ16cm5%ȩŨԼɫһӣɫߵӦһʯ-(855)չ16cm24-ԼɫһȺɫߵȩӦ

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1.桷㣬أרһгҡޡסʹɡʪξ𣬴ϳͷ֮Чǣҩ߳θ뾭ҲصаЧϸ˽ζУζʣ¶ң֮ǡҶζͬư˽Ǵʶ֮ζνܳ䣬аˮãþ2.Ʒ㾫Ҫһʹ3.ܡСۣɡʪʹθֹŻʳŲ㡣()ҡ4.ҽѧšרʹ5.ڼⶾ6.ҽҪľ³

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