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ҽҩȫ > ҩ > ҩ > G > ɽ

ɽ

2017-06-15 11:48 : Ķ:

йҩ䡷ɽ

ҩơɽ

ƴGàn Jin

ԴƷΪֲZingiber officinale Rosc.ڣȥɳɹɻ¸Ƭɹɻ¸߳Ϊ“ɽƬ”

״ɽ:ʱƽ״ָ״֦37cm12cmһɫdzɫֲڣƼԵĻڡ֦Ҷд棬֦оۻѿʼʵưɫҰɫԻԣƤ㻷ԣάɫ͵ɢڡ㡢죬ζ

ɽƬ:ΪлбƬָ״֦16cm12cm0.20.4cmƤһɫdzɫֲڣƼԵĻڣһɫҰɫԷԣɼ϶άеijë״ʼʵάԡ㡢죬ζ

𡿣1 Ʒĩɫڶ࣬ԲΡԲΡ״ΡԲλ򲻹Σֱ540μm״λڽСˣҲгѷ״ߣеԡϸ֬ϸɢڱ֯Уںɫ͵λ򰵺ɫʡάɢ룬ȶ˶ۼ⣬ֲ棬еһ߳ʲ״״ֱ1540μm,Ժ񣬷ľбϸƿףɼƱĺơƼƵܶΪƵܣֱ1570μmܻάʱɼںɫĹ״ϸֱ1220μm

2 ȡƷĩ2gҴ20ml20ӣ˹ҺɣӼ״1mlʹܽ⣬ΪƷҺȡɽҩ2gͬƳɶҩҺձɫ׷¼ B飬ȡҺ4μlֱͬһȼ׻άΪϼĹ轺GϣԻ-ѣ1:1ΪչչȡɣȩҺ105ߵɫƷɫУҩɫӦλϣͬɫİߵ㡣

ⶨȡƷַˮ700ml ջӷͲⶨ¼ DⶨƷӷͲ0.8ml/g

ơɽ:ȥʣݣϴ͸кƬ飬ƷΪƬ״0.20.4cmܻҷֵķⶨù5.5

̿:ȡɽ飬ճ̿¼ Dɫڲغɫ

ζȡ

龭Ƣθġξ

Ρɽɢͨʪ̵丹ʹŻйк֫΢̵ȡ

÷39g

ءﴦ

Ƽࡣ

ժ¼йҩ䡷

ɽ

ҩǵ䡷ɽ

ҩơɽ

ƴGn Jin

׽Ŀ

ԴΪֲҶήʱȡȥҶɳɹɻ΢ɡ

ԭ̬̬""

ֲȫ󲿷ֵвĴݵȵء

״ΪƽĿ״ָ״֦46ף0.42סҰɫһɫֲڣƼԵĻڣڷ֦Ҷд档ʼʵԣҰɫ򵭻ɫԽϸС͵㼰һԵĻơ㣬ζʼʵƤһɫڻҰɫ㡢ٽΪѡ

ơɽʣˮ36Сʱ̳ƣƬгС飬ɹɡڽȡ飬ù𼱳ݹƤʽɫڳʻɫˮȡɹɡ

ζȡ

١"ζ¡"

ڡ¼"ȣ޶"

ۡҩۡ"ζ"

龭Ƣθξ

١ݾ⡷"ΡΡ"

ڡ䱾ݡ"ɽ̫֣ڽ̫Ѫ֡"

Ρ𺮣ͨĸʹк֫΢δȣ纮ʪԣ¡Ѫ

١"УֹѪʪԣº"

ڡ¼"κ丹ʹж񡢻ҡаƤֹѪ"

ۡҩۡ"䣬Ѫȥ磬ͨ֫ؽڣȥ綾ԣҹС㡣ԣУҲֹʹѪա䣬˼֮"

ܡƱݡ"η磬ֹѪ΢"

ݡջӱݡ"̵תк䣬θŻѪֹǺ飬ȶθʳ"

ޡҽѧԴ"Ҫƣͨȥอ֮θкʹ"

ùţ"ºƦĿࡣ""Ƣθ"

ࡶҽѧš"ڽƢθﺮˮйݣűңĸʹֹѪѪ©"

ᡶҩƷ塷"ڽȡ"

⡶ɳҩ⡷"ʪУǣ³棬ƽԣݣֹй"

÷ڷ0.51.5Ǯ

ע⡿ȡѪ߼ɷи

١ݾע"ؽΪʹˡʸɱġݹж"

ڡݾ衷"÷ĿȣѪȺԺ඾ѪŻ񣬻ȸʹ֮"

ʹɽĩ¾Ʒذ߷ȱ󷽡

ȣﺮȣ棬΢񺮣ɫ࣬ʹŻʹֹߣʲݶˣӴһöãȥƤưƬɽǿ˿ζˮȡһϣȥңٷ˺ۡͨ

кˮкɽڣĩǮǧ𷽡

ͷĿ棺ɽڣʲһ˳ɫ϶ζΪĩÿġǮˮյ˷֣ʳǰȷ÷ֹ

Ż²ֹɽ˲θһĶζĩ֭֮Ϊ裬Ӵÿʮ裬Ҫԡɽ˲ΰ裩

˺֮󣬸շ겻ߣҹŻʣޱ֤΢̫ߣɽһһöãȥƤаƬζˮȡһȥңٷ˺ۡɽ

Ƣűɽȷ֡ΪĩÿһǮˮһյ߷ַɽΪĩٷʱ¾ƷǮذ̨

κɫɽж󣬺öҹһȱ󷽡

ѪֹɽΪĩͯСһǮǧ𷽡

¡Ѫ顢˷֣֣ܺѻһǮҶһǮ֣̿߷֡ˮ壬ٲ˪ĩ۾۷ҪϺ

Ƣθʳ٣ѻݣɽƵУ԰п飬ˮԡܻӴÿʮ衣ʮ

֮أ䣬ˮУˮ״ʣСʳʣ½ʹǧǮʲݡɽ߸ζˮȡҪԡɽ

αۣ׽ĩˮġռ÷

Ӹҳ𣺸ɽһϣĩ׵Χͷ֢ɡ

ժ¼ҩǵ䡷


лݡɽ

ҩơɽ

ƴGn Jin

ӢDried Ginger

׽

ԡũݾ

ԴҩĻԴΪֲェĸƷ

ֲﶯZingiber officinale Rosc.

պʹأ10Ѯ12ѮҶήʱȡȥҶɡȥɳƤᄏɡ

ԭ̬ݱ50-80cmʺ񣬶ưɫŨζҶų2УޱҶ೤2-4mmҶƬ״Σ15-30cm1.5-2.2cmȶ˽⣬Ҷ״ëԸг15-25cm״ԲΣ4-5cmƬΣԼ2.5cmɫԵɫȶСͷܳԼ1cm3̼ݣڻɫܳ2-2.5cmƬ3Σ2cmмƬԲ״ѿϻƬ̣ɫƺ͵ɫߵ㣬ƬΣɫɫԵ1ɫҩԼ9mҩסӷ3ңë1ͷΡӶɫ8¡

ֲҹвϲϲٹΪࡣ

ࡿڵԴб㣬õɰأ2Ѯ40-50cmӲݡ4³ٷ1-2Σƽֲ3Ѯֽɹ2-3գȻϣ¯£20-30ңٽѿԼ3-4ܣѿ1-15cmʱȡֱһѿһгС飬ȼ֡С45-50cmѨ10-15cmÿһ飬ѿϣֺʩ򣬲ľҵȷϡ

״״ ʲ״Ա⣬ָ״֦3-7cm1-2cmɫdzɫֲڣƼԵĻڡ֦Ҷд棬֦оۻѿʼʵưɫҰɫԺͿԣһԲƤ㣩㣨άɫ͵ɢڡ㣬죬ζ

ʼʵɫưס㡢ζŨΪѡ

΢棺ľΪбƽľϸƤɢжҶάƤԣɼϴռĴ󲿷֣ɢжάʴάСнϽܣľʲڲΧзľάƷ֯ɢϸϸ

ѧɷ֡ɽͺӷԳɷ֣α-ϩ(α-zingiberene)ţ(geraniol)β-ûҩϩ(β-bisabolene)Ȼ(nerol)18-Ҷ(1,8-cineole)α-ʹ(α-terpineol)(borneol)β-ˮϩ(β-phellandrene)(linalool)׻ɻͪ(methylnonyl ketone)ϩ(camphene)ϩ(limonene)ˮϩ(sesquiphellandrene)α-ϩ(α-curcumene)(menthylacetate)70֣ɷ֣6-(6-gingerdione)6-ͪ(6-shogaol)8-ϩͪ5-ȥ-6-(6-paradol),6-(6-gingediol)6--5-(6-gingediol-5-acetate)6--3-(6-gingediol-3-acetate)6-˫(6-gingediacetate)6-׻˫(6-methylgingediacetate)ȣɷ֣ϩͪ(gingerenong)ABC콪ϩͪB(isogingerenoneB)⽪(hexahydrocurcumin)-35--17-˫-(4- -3-)-[35-diacetoxy-1,7-bis-(4-hydroxy-3-methoxyphenyl)-heptane]35--1-(4- -35-)-7-(4-ǻ-3-)-[35-diacetoxy-1-(4-hydroxy-3,5-eimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-heptane],(3S,5S)-ǻ-1-(4-ǻ-35-)-7-(4-ǻ-3-)-[(3S,5S)-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-heptane](3S5S)-35-ǻ-17-˫-(4-ǻ-3-)-[(3S,5S)-3,5-dihydroxy-1,7-bis-(4-hydroxy-3-methoxyphenyl)-heptane],5-ǻ-7-(4ǻ)-1-( 4-ǻ-3-)-3-ͪ[5-hydroxy-7-(4-hydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone]35--7-(3,4-ǻ)-1-(4-ǻ-3-)-[3,5-diacetoxy-7-(3,4-dihydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-heptane],5-ǻ-1-(4-ǻ-35-)-7-(4)-ǻ-3-β-3-ͪ[5-hydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-3-heptanone]5-ǻ-7-(4-ǻ-35-)-1-(4-ǻ-3- ڱ)-3-ͪ[5-hydroxy-7-(4-hydroxy-3,5-dimethoxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone](3R5S)-3,5-ǻ-17--(4-ǻ-3-)-顢(3S5S)-3,5--17-˫-(3,4-ǻ)-[(3S,5S)-35-diacetoxy-1,7-bis-(3,4-dihydroxyphenyl)-heptane]7-(34-ǻ)-1-(4-ǻ-3-)-4-ϩ-3-ͪ[7-(3,4dihydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-hept-4-en-3-one]ȡ

ɽ6-(6-gingesulfonic acid)5--ǻ-2-O-β-D-߰(angelicoidenol-2-O-β-D-glucopyranoside)֬(gingerglycolipid)ABC

ҩá1.ϵͳ1. 1ԴθճĤϸı1.1.10.6NθճĤ˾ԵıãʵΪٶ͢ʹ顣ȣʹ5mg/kgˮʱƣig١igˮ2hڢig1ml10%igͬˮˮpHΪ6·015Ӻ3鶯ֱig1ml0.6NᣬlСʱÿθճĤܳȣmmθ˳ϸ״ԣ0-4飨n=9θճĤΪ71.22±13.33ֵ±׼ͬ˵Ϊ2.11±0.42飨n=8ֱΪ23.75±8.390.83±0.30ʹ飨n=6ֱΪ106.60±11·562·83±0.30˵ԣȾP0.05

1.1.2ӦԴ̼θ˵ Ϊ3飬ٶ顢͢ʹ顣֫˿ϣ20ˮУˮƽͻ鶯ˮ֮ǰ2Сʱʹ5mg/kgig٢igˮڡ鶯ڷˮ֮ǰͷˮ36Сʱֱig2ml10%1igˮ9hÿθճĤ˵ԡ飨n=6˵Ϊ17·16±3.20˵Ϊ4.00±0.00飨n8ֱΪ7.37±2.672.75.49ʹ飨n6ֱΪ37.49±6.96ȽϣP0·05

1.1.3ŽθڵӰ Ϊ۲θճĤıǷθйأ£ʸţȻϸڣig2mll0%飩ˮ飩4Сʱ󽫶ﴦʸţθȡθ俿ŴһСڣθҺռڶĹڡⶨ4hθҺȣų10%2mligɴ̼ŽθҺķڣθҺȺųȶմӣP0.05

3ʵpo10%Ž0.6NˮµĴθճĤˡ䱣û̼θճĤϳɺͷžϸõԴPG¡ʹʹθԴPGϳɺθճĤıüʧ10%ɴ̼θķڣͨPGĻñϿƺìܣʵͳһģ̼θҺԴٽܡͬʱֱθճĤθáںϳɵPG٣ϳɶθõPGδʾPGθڵãθճĤıáθճĤϸPGԶСθ

1.2-Ҵθ ͪȡ1000mg/kgɳθճĤãΪ97.5%塣ͪȡһù轺õϩpo轪ϩ25.50mg/kgɳθճĤãʷֱΪ80·3%98.7%ͳѧ塣ʮָ轪ϩ2550mg/kgŽδθճĤáˮӦоpo轪ϩ2550mg/kgҲδθճĤá-ҴЧҩЧɷΪϩθճĤϸá

1.3øõӰ ø2ֵøֳǿ谭áøԵۡ׺֬нǿƻøеĵøʹøԵ۵½ƵøBø谭ǻ

1. 4Ը𺦵ı1.4.1ԴȻ̼𺦵Ӱ ȡ100-145gֳ顢˶ʵ12顣3鶯ʵdl15%Ȼ̼0.2ml/100gig1Σ24Сʱ͸𺦶1%һ80ˮigʵ12ֱ0.32ml/kg0.4ml/kgigÿ12գĩθҩ16СʱոʱͷȡѪѪ壬ȱתøSGPTȻ̼ж24Сʱʼƣҩ2dʹϸٻָܽȻ̼жѪSGPTֵʾȻ̼¸á

1.4.2СȻ̼𺦵Ӱ ͣig0.5mg/kg·d5dȻd5ip0.1%Ȼ̼10mg/kg16h󣬿ոժȡѪѪ壬SGPTֵͶȻ̼¸Ԥá

1.4.3С̪ƣBSPӰ ȡС30ֳֻ3顣1Ϊ飬23ֱΪ𺦶鼰顣ʵd1d2120.1%һ80ˮ10ml/kgig30.25ml/kgigÿոҩΡD3磬԰ҩ1ΡD3磬1԰ǰҩ230.1%CCl410ml/kgipڴСivBSP200mg/kg20ӺժȡѪѪ塣ȡ0.1mlѪ0.1NHCl5ml520nmɫȻ2NNaOHһΣٱɫǰնȶֵOD?BSPСCCl4жBSPߡŽжСBSP˽һ֤ʵ鶯֮𺦽ᡣ

1.4.4۷ҺʵԸ޸ܵӰȡ۷ۺʽϴȥƤ飬˫ɴˣȡ֭ۻȣմɹܷ⣬ڵԼ1m10dȡáʵ鶯Ϊ顢顣ʵdld5ֱ鼰scȻ̼0.5ml/100gأһΡͬͷעˮʵld翪ʼԽ۷Һ0.5ml/100gأigÿ1Σ7գͬͨˮigʵ8dȡѪⶨѪתø̬ѧ۲졣SGOT½壨P0.05ĸСҶṹ˳̶Ƚᡣ⽪۷ҺԴ60%ҴʵԸβбá

1.4.5ȡWistarԼ250g̹ʸþϩܲܵܣÿֻ󱣳lСʱʹ֮ȶ30Ӻʮָҩڸҩ0.5,11.52345СʱⶨڵıͪȡҺڸҩ3СʱŵãˮҺЧ6-ڸҩ30-60ӿʹ֭ӣڶԱȵIJοҩ⵨Ƶãڸҩ4СʱԺԣ10-Ҳãýԡ

2.ѭϵͳ ivϩ500ug/kg۲⵽ѪѹһԽͺԺֳ½áһԽѹΪжơĵͼϩӶԴ̼ϵͳĴãÿ뽪ϩӵĽѹòءϩӵѹòжϼѪƽ5mg/kg20СʱǰѪܽȫʧԳؼиڽϩӣ1-10ug/kgѪѹں֫ѪܴʵУϩӵѹÿ༰ĩҲءں֫ѪܹʵУȫѪѹģ˲ivڸҩоϩӣl-10ug/kgʱеļѹãеļѹãѹΪƣѹΪжơЩϩӾ˷񾭼ĽѹáĩѪá˷õȲֵزѹá˿ڽ1g£ʹѪѹ䴼Ѫ˶༰˷áҲֱ˷ãʹѪţٽѪҺѭ

3.񾭵3.1ԷӰ СΪ3顣ʵϵҹСԾʱYSD-4ҩǼҩǰ¼5ԷʵСֱip0.120.19ml/kgõˮڸҩ3060ӷֱⶨ5СԷзdzŵԷ

3.2ͱ˯ʱӰ Сֱip0.120.19ml/kg͵ˮҩ30ӣ鶯ipͱ25mg/kgԷʧָʱΪ˯ʱָ꣬ӳͱƵ˯ʱ䡣

3.3˷ҩ¾õӰ Сֱip0.240.3ml/kg͵ˮ30Ӻ󣬸ٷֱipĵ100mg/kgӡ7.5mg/kgʿ1.5mg/kg¼120minڵľ18ԶԿĵʣӡغʿľ޶Կá

2. 4ʹ3.4.1Ť巨 Сֱig0.3mg/kg䶡40mg/kg͵ˮҩ30ӸСip0.3%0.2ml/ֻͬʱ¼20ڵŤ巴Ӧ19ʹã϶䶡40mg/kgΪ

3.4.2Ȱ巨 С30ֻ3飬ں55±0.1£СΪʹӦָ꣬ҩǰʹ2Σÿμ5ӣֵ>3S60s߹ʵáСֱig0.4ml/kg䶡40mg/kg͵ˮҩ306090120Ӹ1ʹ60sʹӦ߰60s㡣20ŵʹá

3.5ԽĸȴµӰ ʵơƽĸҺ24ֻ漢Ϊ3飬ơƽĸˮ20%Һ2ml/100gscע5Сʱⷢȸ£ٷֱ𰴱20ҩԺÿ1Сʱ1Σ5Ρ0.375ml/kglh󼴿ʼнã5Сʱ

4.ɽȡƤʹõӰ4.1СӰ С10-12gΪ飬⻯scҩ⣬Ϊigҩÿ1Σ7dd8ժȡ٣Ťƽأհ׶Ƚϣt飬ɽ4.0g/kgʱʹСήΪ50.7%P0.01

4.2ԴάCӰ ɽ͸ɽȡ100-120gάCԽ͵á

5.ɽȱܺСӰ ɽ÷г30-60ʯȡ2Σÿ3dʯѣûƺɫ״ɽʯȡƸɽҩпˮ0.5Сʱ2Σ1ml1gҩĸɽˮ

5.1СѹܱȱӰ С60ֻ220±2.5gΪ6飬ֱigɽͶҺ1СʱΪ145mlĹƿڣⶨƿ21%l5%10%17%ʱ䣨ٶȣʱ估ʱƿڲдɽܼСĺٶȣӳѹܱȱСĴʱ䡣

5.2ԶͷСſڶʱ̵Ӱ С60ֻ19.2±2.4g6飬ֱigɽȡͶҺ1СʱԾԴ¼ͷСſڶʱ䣬ɽӳſڶʱ䡣

5.3ƣNaNO2жСʱӰ С22·6±2.0g6飬ֱigɽȡͶԡҺ1СʱNaNO2800mg/kg¼ʱ䣬Ϊ1.5ml/kg3.0ml/kgˮΪ10-20g/kgʱԲ𣬲ӳʱ䡣

5.4ܺСʱӰ С72ֻ19.5±1.3g6飬ʳˮ24Сʱ󣬷ֱigɽȡͶҺ0.5СʱװƤСڣÿ6ֻƤз18--20ڣÿʱ۲1Σȡ󣬲ϲÿ6ֻң۲쵽СȫΪֹɽˮȡӳܺСĴʱ䡣

6ѪСõӰ6.1ADP10umol/LյõѪС6-ڸŨ²ųáԻϩᣨ160umol/LշѪС6-ãЧǿʹƷIC50ֵ6-Ϊ2.2umol/LʹΪ4.3umol/L6-԰ԭշѪСҲãIC50ֵΪ24umol/LȶԻϩЧ1/10ҡ

6.2ˮĿۼ ˮѪСбAAĴлӰоTXB2PGsż١ˮܽPG-ڹγɡˮTXPGsͬʱִбAAϳ6-ͪ-PGF1aγˮĴڶ١ˮﻹܼʹȽбAATXB26-ͪ-PGF1aˮǿѪСۼãСˮҲAAյľۼáˮPG12ĺϳɡ

6.3PGI2Ӱ ADPշѪСΪָ꣬о6-ԴƬPGI2Ӱ졣·ƬҺADPѪСԵá6һ36360umol/L붯Ƭͬʱ·ADPѪС·ƬҺõķӦȳԵĴٽãŨȶ졣⣬Ѱ˹ƥ֣560umol/L붯Ƭ·ҺҲܴٽADPշѪСķӦȽ·ƬҺ巴ӦΪš

7.PGϳɵӰCollierHOJȱзǻһЩߴ̼Եҩд̼PGϳøáͪZingeronePGϳɵĴ̼ãPGE2׼㣬SC50±seŨΪ0.041±0.002mmol/LЧŨΪ0.52mmol/L

8.8.10.25-0.4mligС鰷ʹСëϸѪͨ͸ԵӣԶױС֢ʹãѿ֯ʹӣƴһƤϵͳĹܡ

8.2Ի֬øϵͳӰ 6-3.6-360umol/LԻ֬øĻԳŨԵơ6һTXB2IJҲŨԵáƷʹ28-280umol/LҲԻøTXB2IJŨԵá6-Դ΢廷ø6-ͪ-PGFaҲŨԵáIC50ʹȣʹΪ1ʱ6-Ϊ0.386-0.3-100umol/LŨ¶5-֬øŨԵãIC50ֵΪ1.6±0.6umol/L6-֢ҶԱ̬ӦЧ

9.ԭ ˮ˺˾һɫѢγв̶ͬȵáзֹѪѷãرȡкͪԳɷʱøǿýۼˮƬͪɷֵƬѪ没ʹѼ½Ѫһɱá

10. кĽʹĩijЩͷţʹԪͷųPʣءøġѪܻԳĵȡôСԻǰߵմٽýоˮŵմٽãǿöȡ״ȡõIC50%ΪTBAVThiobarbituricacidvalue2.34x10-2POVPeroxidevalue3.04x10-2

ԡСipLD50Ϊ1.23±0.11ml/kgP=0.95igLD50Ϊ3.45±0.28ml/kgP=0.95СǰȺֻ١ʧɳۡԡӴףС1igɽʯѣ30-60棩ȡ۲7dLD50Ϊ16.3±2.0ml/kgp=0.95С20ֻigɽˮ120gҩ%۲7dɽСSCҩLD50Ϊ33.5g/kg

𱡲ɫףȡɽ1g5gĥ飬Ӽ״ҡ1h˹ҺŨԼ1mlҺԷ18-ΪƷֱͬһ轺Gϣʯһ8515չ1%ȩҺɫҺɫƷɫ׵Ӧλϣͬİߵ㡣

Ʒʱ־л񹲺͹ҩ䣩1995涨ƷӷͲ 0.8%ml/g

ơɽʣˮ3-6Сʱ̳ƬгС飬ɹɡڽȡ飬ù𼱳ݹƤʽɫڳʻɫˮȡɹɡ

ζζ

龭Ƣθģξ

Ρɢͨ·λ丹ʹŻ£йк棻ȣʪʹ

÷ڷ3-10gɢãϴĩ

ע⡿ȡѪ߽

1.Ԫأɽ֮Կֹ࣬ƣﺮ,жֹҲ֮ߣҲ2.꽣ɽڿ࣬Ҳаθ,֮ɢԪ,ɢ֮,׳ʳҲ,ʲݻ֮ɢﺮ,ͬζ·,ͬ˲θҲ

3.ɽʪξѪҩѪͬҩѪҩѪѪ鷢ȡߣֹ֮ѪѪ보֮Ѫɫ׶زߣ˴Ҳ˸ɽ֮Ѫ¾

4.ĿɽѪҩѪ֡ҩ֡ȥ£֮⣬Ѫ֮ѪѪѪߣ֮ã֮Ҳ

5.ݾ衷ڽɢаᣬ¿ɳͨгʪԣں䣬ֹʹֹѪߣѪȣѪУɽڣѪҩ,Ѫò,ѪӡγƧ,Ҳ6.Ԫ䣬Ϊкר²ߣԸɽ֮ȣʢѪѪߣ֮̿ʧӡֹѪߣɬ֮Ѷʢ𲻹Ԫ鲻ѪΪѪѪѪߣ˳֮ΪֹѪ֮Ҫҩ

7.ҩƷ塷ɽɾãйζȹֹ֮Уרɢﺮ縹ʹкȲԸʲݣȡʺϻΪ֮塣ɢɢ꺮Сʹңֹкͨл֮з֮ɢɽأһı......ڽȡ

8.ݳԭũֹɽڽԸɽںڣν֮ڽҪԡηøʲݸɽɽڣڽ֮ã֮ӡζڹζԼβѪȣﺮѪѪѪ֤̫֮ʲ棬ν֮̿ζ΢಻ḡʵֲڽ֮ӡڽ鿪֮ĸʼΪ

9.桷ɽ޶ضߣθ䣬ԪԸͬͶܻЧи޽֮䣬پ桢֮ͨͬζͨκԣͬʪƢͬѪ......10.УֹѪʪԣƧ

11.¼κ丹ʹж񡢻ҡаƤֹѪ

12.ҩۡ䣬Ѫȥ磬ͨ֫ؽڣȥ綾ԣҹС㡣ԣУҲֹʹѪա䣬˼֮

13.Ʊݡη磬ֹѪ΢

14.ջӱݡ̵תк䣬θŻѪֹǺ飬ȶθʳ

15.ҽѧԴҪƣͨȥอ֮θкʹ

16.ùţºƦĿóࡣƢθ

17.ҽѧšڽƢθﺮˮйƧűңĸʹֹѪѪ©

18.ɳҩ⡷ʪУǣ³棬ƽԣݣֹй

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