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ҽҩȫ > ҩ > ҩ > G > ʲ

ʲ

2017-05-29 22:30 Ķ:

йҩ䡷ʲ

ҩơʲ

ƴGn Co

ӢRADIX GLYCYRRHIZAE

ԴƷΪֲʲ Glycyrrhiza uralensis Fisch.͹ʲ Glycyrrhiza inflata Bat. ʲ Glycyrrhiza glabra L. ڣȥɹɡ

״ʲݣԲΣ25100cmֱ0.63.5cmƤɽһɫɫŵơơƤ׼ϡϸۡʼʵάԣưɫԣγɲ㻷ԣ߷״е϶ԲΣѿۣв衣΢ζ⡣

͹ʲݣľʴ׳еķ֦ƤֲڣɫҺɫʼӲľά࣬Сѿִ

ʲݣʵؽϼʵеķ֦ƤֲڣɫƤϸԡ

𡿣1 Ʒ棺ľ˨ΪɫϸƤխƤ߿㣬϶άľ΢ľΧϸƷɸȺѹΡγɲԡľʲ߿35ϸܽ϶ֱ࣬Լ160μmľάΧϸຬƷ裻衣ĩػɫάֱ814μmں΢ľΧϸƷγɾάƷԵƿ׵ܽϴϡƵܡľ˨ϸɫΣ΢ľ

2 ȡƷĩ1g40mlȻ1 Сʱ˹ҩӼ״30mlȻ1 Сʱ˹Һɣˮ40mlʹܽ⣬ȡ3 Σÿ20mlϲҺˮϴ 3ΣɣӼ״5mlʹܽ⣬ΪƷҺȡʲݶҩ1gͬƳɶҩҺȡʲ臨ƷӼ״Ƴÿ 1ml2mgҺΪƷҺձɫ׷¼ B飬ȡҺ12μlֱͬһ1ҺƱĹ轺DZϣԴᣭᣭˮ15:1:1:2Ϊչչȡɣ 10ҴҺ105ߵɫƣ365nm ¼ӡƷɫУҩɫӦλϣͬɫӫߵ㣻ƷɫӦλϣͬijȻɫӫߵ㡣

ⶨոЧҺɫ׷¼ Dⶨ

ɫϵͳ ʮϹ轺Ϊ״-0.2mol/LҺ-ᣨ67331ΪࣻⲨΪ250nm۰ʲᵥηӦ2000

ƷҺƱ ȡʲᵥζƷԼ10mgܳƶ50mlƿУܽⲢϡ̶ȣҡȣãÿ1mlʲᵥζƷ0.2mgۺϸʲΪ0.1959mg

ƷҺƱ ȡƷзԼ0.3gܳƶ50mlƿУԼ45ml,250WƵ20kHz30ӣȡ䣬̶ȣҡȣ˹á

ⶨ ֱȡƷҺ빩ƷҺ10μlעҺɫǣⶨá

ƷʲᣨC42H62O162.0

ơȥʣϴ͸кƬ

ζʣƽ

龭ġΡƢθ

ΡƢȽⶾ̵ֹȣֹʹҩƢθ뵡ļ̵̣࣬丹֫μʹӸ״ҩﶾԡԡ

÷1.59g

ע⡿뾩ꪡܾͬá

ءͨﴦ

ժ¼йҩ䡷

ʲݵͼƬ

ȫвҩࡷʲ

ҩơʲ

ƴGn Co

ݸʲݡ۸ʲݡ۲

ԴΪƸʲֲʲGlycyrrhiza uralensis Fisch.״ҰƷ^ڣƷڲ34^ڣȥоϸֱɹɡ

ԭ̬ݱ30100ס׳ԲΣζƤɫɫֱľʣɫëʹë״塣״ҶҶԼ6ףҶ䣻СҶ717Ƭ״ԲΣ25.5ף13ףȶ˶ԲԲ汻弰ëҶҸ״򣬻ܼ״ëʹë״壻λڵɫ1.42.5ף󣬾״ԲΣжצ꼰ǰгצԹ״ԲΣܼʱ״״69ףܱɫë״壻28ԲλΡ

ֲڸԭɽ¡ֲڶࡢຣ½ɽȵ

ࡿϲɺɰɰشڵˮλߵĵ֡ӷֳѨɣǰ30ҵˮ23Сʱ֡о1ߣѨ5翪ѨѨ2磬ÿѨ5105֣ÿĶ35ڼ䣬⣬˹ཽˮ׷ʩõ׷Ϊ

ѧɷ֡״иʲأglycyrrhizinʲglycyrrhinic acid,C42H62O16614Ϊʲݵζɷ֣һ߰ʲˮһӸʲݴᣨglycyrrhetic acidglycyrrhetinic acid,C30H46O4ȩᣨglycuronic acid,C6H10O7ʲݻ߰ʲ߰liquiritin,C21H22O9,Ϊһֻ鴼flavanone߰߰Ԫʲliquiritigenin,,C15H12O4͸ʲݿ߰glycyamarinʲݻ߰iso-liquiritin,,C21H24O9ǻʲݴᣨdihydroxyglycyrrhetic acid grabric acid,C30H46O5ʲlicoricidin,,C25H32O5,3’6-ϩ2’4’5-ǻ飩ʲݴglycyrol,C21H18O65-0-׻ʲݴ5-0-methyl glycerol,C22H20O6ʲݴiso-glycyrol,C21H18O6⣬ки¶mannite3.82.46.5ƻᡢľᣨbetulicacid,C30H48O3춬ᡢأbiotin,C10H16O3N2S296΢/ˡ΢ӷΪʲгԴ۵ȡ

ҩá1.ⶾãʲػнǿĽⶾã԰׺ء˷綾нǿĽⶾãһЩԼʵԸס඾߶нⶾáⶾûƿǶ෽ģͨѧʽijϣǿĽⶾԼʲصˮȩҲǽⶾõЧɷ֡

2.׼̬ӦʲݴԴѿסȩԸף˾طӦƤѿ֢áʲ李ʲݴЧӰƤѿ֢ڼڣǿڻӽڿɵɡʲĸƼ֮ãεĻԽϸߣ󡣸ʲݿ׿̬Ӧԭδȫ

3.ʲ̵ãܴٽʺ֧ܵķڣʹ̵׿

4.ʲݴܻèʵԿԾá

5.ʲݵĸƼԴʵθԵáʲݵˮбθճĤθáٴҩоҹ۲죬ʲˮθճĤϸ“ǰ”ɷ֣ʹθճĤ˺

6.θҺڵӰ죺ʲθθᣬܽθŨȣպҲܷáԻá

7.⾷ʲݼԶ峦ܾãȻ֯ijн⾷áʲݶԶ峦ܼθɳá

8.ʲݶڶʵԸˣʹԺͻԼᣬϸĸԪǺẬ󲿻ָӽѪȱתø½ʲݾп˵á

9.ƤʼãʲʹֶƵų٣ųӣѪѪƽͣƤСήʲʹ17-Ƥ̴йӣͼ٣Сήӣ״ȼӿάC͵ȡʲǿӳɵáʲݲƤʼõԭΪʲݴĻѧṹƤʼƣҲƣϵһֱãҲΪһּüʲݴƤʹ̴ڵƻѪҺƤ̴ӦӣֽԵƤʼá

10.ʲжԿãǿصǿá

11.ʲݴڴʵԹˮáСϸˮá

12.ʲܾ෴ã߹ĶԽϷֽߣܾʲͬãкܵƣǿʲݶԡ

ơ˸ʲʲƬķˮȺƣŹڳɫͲճʱȡÿ1002530

ζʣƽ

ΡȽⶾֹȣҩ˸ʲܲƢʺʹԣƢθθʮָ񣬸ף񯲡Ӹ׶ҩPʳж

÷0.53Ǯ

ע⡿졢ꪡܾͬá

1.θʮָ񣺸ʲ10ˣ15ˣҶ0.5ˣϸۣÿ3ˣÿ3Ρ

2.񯲡ʲ5Ǯ1С4Ǯˮ

3.£ڲ˸ʲݡΡء󶬡ʸ3Ǯ֦1.5Ǯ3Ƭ5öȣҹ²ȥ֦ʯ5Ǯĵ1߼ӻ3Ǯζ1.5Ǯ

ע1.ƷʲҪʲʲ࣬ʲڶɹŶԭֲҪϵʲGlycyrrhiza uralensis Fisch.ʲɹϲຣɽԭֲҲǴ֡

2.ʲ֮½ģʲ⣬У

1ʲGlycyrrhiza glabra L.ֲ½ɷʲơ

2͹ʲGlycyrrhiza inflata Batal.ڵʲݼʲݾʲҩֲ½

3ƸʲGlycyrrhiza kanscensis Chang et Pang, mss.ֲڸ༰½ʵؽϸʲմΡ

ժ¼ȫвҩࡷ


ҩǵ䡷ʲ

ҩơʲ

ƴGn Co

ݡ۸ʣ۲ݡʀݣ¼ϣպ뾰ͨ顷۲ݣȺסݣйҩֲ־ӣҩ־ݣҩ

ݡ

ԴΪֲʲ״^ڣȥ֦桢ȣسʵ̵ĶΣɹɣСɹȫɡҲн˨ƤȥߣΪ"۲"øͨ紦ù

ԭ̬ݱԼ3070ף1ףԲ״ִƤɫɫֱԴľʣɫëۻ״ë״ҶҶΣ䣻СҶ48ԣСҶ̣1СҶƬԲΡ״ԲλżԲΣ25.5ף1.53ףȶ˼״ͨԲΣ汻ۼë״Ҹܼ512ףΣԼΪڵ12Գ5ΣͲԳⱻëۣڵɫԼ14˺ף󣬳ԲΣȶԲ΢ȱ²жצǰֱ̣гצ102壬˿̲һҩСȣ1ӷޱԹ״ԲΣ״ʻ״ͨ68ףܱɫĴ״ë28ԲλΣɫ⻬67¡79¡

ֲĸʲԭӰɰȵءֲȵءɹšࣻΪɽ֡ӱຣ½ȵءɹ˺Ʒš

״ʳԲΣ֦سɳ30120׵ĶΣֱ0.63.3סƤĸʲݣƤɽȣԺɫɫɫŵơƼϡϸۣƤ׺΢ͻ𣬳ʰɫƽ룬¡ʼʵءάԣưɫԣһԵĻƺ;ջģγ϶.΢ζ⡣״״ƣѿۣ衣۲ƽ̹ɫάԣơƤʲƤϸ幵ɫʼʵ㡢ưɫΪѣƤֲڣɫɡСɫΪΣƤغɫʼӲػɫζ߲ҩ۲ݽϴƤʲΪѡ

ѧɷ֡ʲݸʲأΪʲᣨglycyrrhizic acidļءΡкʲ߰liquiritinʲ߰Ԫliquiritigeninʲ߰isoliquiritinʲԪisoliquritigenin¸ʲ߰neo-liquiritinʲ߰neoiso-liquiritinȡ

ҩáʲݴԴֲOberlingGuerinãʲᵥΡʲݴƼʲݴܻ֮СϸˮãʹڷЧʲءʲ߰ԴˮΰСϸˮϸ̬ܲѧϵı仯ʲƤֲļ

ơʲݣȥʣϴˮ˳͸ʱ̳͸Ƭɡ˸ʲݣȡʲƬķ뿪ˮȣƣùĻΪɫճΪȣȡÿʲƬ1002530

١׹١"ʹʲݣȥͷβ⴦þƽɣϸʹһͿˣ־Ϊȡ"

ڡĿ"˸ʲݽóˮպʪ֮ȥƤýˮȡ"

ۡ䱾ݡ"װ賴á"

ζʣƽ

١"ζʣƽ"

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ܡҡ"ʣƽ˸ʣ¡"

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١Һݡ"̫"

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ΡлΣⶾҩãƢθʳ٣ʹ磬;뷢ȣԣļ£ãʺʹӸҴ񣬽ҩʳж

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ܡջӱݡ"궨ǡˣһ𡢾¡ơͨϣ澫׳ǣȡ"

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ޡҺݡ"η֮ŧѪ¼巢֮ңͬ"

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÷ڷ0.53Ǯ衢ɢãĩˮϴ

ע⡿ʵ֤ͼɷ

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ڡҽѧš"á"

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գʹʲߣ۹һʲݶ϶ζˮȡһȥ·ٷ˺ۡ۹

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жʲݰڶ֭ݡܡ

ǦжʲǮʣȥƤ⣩ǮζһΣ졣195610£

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2ʲˮֲʪ2Сʱ1Σÿ1520ӣƽӴƤ12һ14켴ˣҺֹͣϣп¯ʯϴռøʲ1ˮϴÿ1ΣԹƤЧʲݴʪţƤѢҲá

ȡʲ1Ƭ75ƾ100ڣ24Сʱ˳ҺĸͺˮϺͿ뻼17֢ߣЧ⡣

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5812ʲҺʲݴҺÿ10ˣ1030ʲݽҺۣݲҪÿ12Сʱ1λÿյ34Σ۲Чף60ԽĤסĤĤ׼״Ĥ׻ߣ27ң56Ȭ2ͣҩıԽĤҩ214죬ʹѪʧϹĤסĤףֱ۲45ҩ317ڽʧ634֢ˡ紺ԽĤף6ҩ23ĤѪᣬͣҩҩܿơ⣬Խ˹ԽĤ׼ĤףЧĤסĤʵסĤףܼѪʹ֢״Ĥı䲻ԣԼԺĤ״ʹѪʹ̼֢״ᣬǰǷпã۲һ¡Ըʲɲãͬʱݣ1ԺĤ״յЧ

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ȡʲݡܾ3Ǯˮ2000ԡϴ˲λÿ3Σÿϴ35Σ֮ϴûɴҩ֡㶳˹76ҩ1858Ȭʵ֤ԵЧѣҩ1ԡϴ35μԺ׼ƤĴ棬ϴʹʧ֮֯䣬ѿ֯ûҩ漴ѸϣԶѴЧѷԡϴʹɸ﷢ӲΪȬȶ˽廵ߣϴʹʹѸʧ֯룬ơ

⣬ԸʲƼΪҩƷ֢סײݡԹؽסѪѹС赸֢۴ס̱ަжľжȣȻ٣ȡýϺЧ

עҪƷ⣬ֲҩá

ٹʲݣŷʲݡΪֲа˪ëëСҶԲλΡԹԲΣֱֲ½

ڻƸʲݣϲ΢ëë.Թ״ͣëֲࡣ

͹ʲݣСҶ35Ƭϡ7ƬëԹԲζֱֲ͡½ȵ.

ժ¼ҩǵ䡷


лݡʲ

ҩơʲ

ƴGn Co

ӢLiquorice Root

ݡ۸ʡ۲ݡʀݡϡͨ۲ݡݡӡݡ

1.

2.¼ʲݣȻɳɽϿ¡²ɸɣʮճɡ

3.պ뾰ʲݣУϤɽƤ֮ʵǡѡл˸ߣ\㳦ߣƣáݼУ硣ϸʲݣϸʵʱá˲Ϊҩ֮ٲߣгҲ

4.ͼʲݣӶݿ֮

ԴҩĻԴΪֲʲݡʲݡ͹ʲݵĸ

ֲﶯ1.Glycyrrhiza uralensis Fisch.2.Glycyrrhiza glabra L.3.Glycyrrhiza inflata Batal.

պʹأ^ڣȥ«ͷ֦سʵ̵ĶɹɣСɹȫɡ

ԭ̬1ʲ ݱ30-100cmܼ׳Ƥɫֱľʣаɫëʹë״塣״Ҷ8-20cmСҶ7-17λΣ2-5cm1-3cmȶ˼ۣԲë壻ҶΣɫë״Ҹܼ״5Σжëʹë״壻ɫ1.4-2.5cmëԲΣצǰֱ̣гצ壬91ԹΣ״״ܱë״4-8Ρ7-8£8-9¡

2ʲ ݱ1mҡ֦Ƭ״Ͱɫë״Ҷ5-14-20cmСҶ9-17,ԲλԲΣ2-40.8-2cmȶ˳΢ȱжëƬ״壻ҶΡɫгҸ״򣻻״5ȵݣɫë곤ԲΣԼ11mm곤Լ9mmצԣǰ곤Լ8mmԹ⣬Σ20-30mm4-7mmëʱԵ3-4š6-8£7-9¡

3.͹ʲݣݱ50-120cmʱ׳ΪľʡֱֲܼӳƬĵƺɫƬ壬ëëë״Ҷ3-16cmСҶ3-7öΡΡԲԲΣ1.5-5cm0.6-2.8cmȶ˼ۣԲΣԵշΪ״氵ɫ߻ƺɫٵ㣬ɫߵɫٵ㣬ʱͿ״йëëСҶ1-4mm״򣻻СϺɫɡԹԲΣС0.8-2cmͣ޻аѣ΢ëС1-7š6-8£7-9¡

ֲ̬1.ĸʲԭӰɳȵء

2.ԭŷ޵к򣬱ǡϸǺҹ½зֲҿڸɺμԻĵء

3.ɳʡ

Դֲ1.ֲڶȵء

2.ֲڸࡢ½ȵء

ࡿѧ ʲݵϲÿĩԽ괺3-4´Ӹϳѿ֦Ҷ5-6֦Ҷï6-7¿9¼Թ졣ϲ⣬Ǹָʾֲѡˮãˮλϵ͵ɰ֣ݺ͵ˮλߵĵֲԻ΢Ϊã

༼ Ӻ͸״ֳԸ״ֳ졣ӷֳǰӦͷ^0.8-1mʩǷʣ1hm237500kgƽ裬1m17cmо30-40cmѨ6cm㲥о15cm×30cmÿѨ5-6ţѹ1hm230-45kgʲƤӲ͸͸ˮԲǰýƤĥƻˮݺʪɰ1-2²ֳ֡紺ڸʲʱѡϸСĸ״س12-20cmСΣÿ1-3ѿо30cm10cm15cm״ƽڹڣƽѹˮ

ܹ ǰ󾭳ʪ2-3ƬҶʱ10-15cm磬ÿݡ2-3Σ׷1-2Σÿ1hm230000-3750kgԸ˷򣬾Ƿʺ׷Ϊ

溦 а׷۲ⲡְ߲ҪΣҶ5-8·粨0.3-0.4ʯϼ溦桢ʲС壬ηҪǽ԰ٳԴûѧҩΡ

״״ 1ʲݸʳԲΣ30-100cmֱ0.6-3.5cmɫɫҺɫԵơƼ᳤ƤףϡϸۣƤɽһݡʼʵأάԣưɫзԣԵγɲ㻷ƺͷ״϶ѿۣж衣΢ζ⡣ƤϸɫءʼʵɫưסΪѡ۸ʲΪƤʲݣ浭ɫƽ̹еơ

2ʲݸʵؽϼʵɫƤϸԡάԣ϶١գζ

3͹ʲݸʴ׳ɫҺɫʼӲ׳浭ɫɫάԣ١ζࡣѿִ

΢ 棺1ʲľ˨Ϊ30ľ˨ϸƤխƤάΧϸƷγɾάƤ߶϶γɲ㲻ԡʲ߿3-5ϸܽϴ󣬵ɢڻ2-3ȺľάΧϸϷ衣Ʒϸ

2ʲƤƽֱ϶١

3͹ʲƤʲϸγ϶

ѧɷ֡1.ʲ ͸߰Ҫһ֣ȳƸʲأglycyrrhizinģϵʲݵζɷ֣1ӵ18β-ʲݴᣨ18β-glycyrrhetic acid2ӵȩᣨglucuronic acidɵĸʲᣨglycyrrhizic acidļκ͸Ρ߰Уʲ߰uralsaponinAB͸ʲ߰licoricesaponinA3B2C2D3E2F3G2H2J2K2ֺͪ໯ʲ߰Ԫliquiritigeninʲ߰liquiritinʲ߰ Ԫisoliquiritigeninʲ߰isoliquiritin¸ʲ߰neoliquiritinʲ߰neoisoliquiritinʲlicoricidinʲͪlicoriconeâأformononetin5-O-׻ʲݱ5-O-methyllicoricidinʲ߰ Ԫ-4-߰[liquiritigenin-4-qpiofur-anosyl1→2glucopyranosideapioliquiritin]ʲ߰Ԫ-74-߰liquiritigenin-74-diglucosideĵ߰vicenin 򣩼68--ǻ۲ءâ߰ononinʲݻͪisolicoflanonolʲ߰Ԫ-4-߰[isoliquiritigenin-4-apiofuranosyl1→2glucopyranosidelicurazid,apioisoliquiritin]㶹໯ʲ㶹glycycoum-arimʲݷӣglycyrolʲݷӣisoglycyrolʲ㶹-7-ѣglycyrin¸ʲݷӣneoglycyrolʲ㶹licopyranocoumarinʲ㶹ͪlicocoumarioneȡֺ5678--4-׻5678-teTCMLIBahydro-4-methylquinoline5678--24-׻5678-teTCMLIBahydro-2,4-dimethylquinoline3-׻-678-[12-a]-3-ͪ3-methyl-6,7,8-TCMLIBihydropyrrolo[12-]pyrimidin-3-oneʲݱ߻ૣlicobenzofuranʲľ֬أliconeolignanβ-޴β-sitosterolʮ飨n-TCMLIBicosaneʮ飨n-hexacos-aneʮ飨n-heptacosaneȡʲϾGBWglucan GBWԵľ״ƤԵĸʲݶǣglycyrriganUAUBUC־˷õĶǣpolysaccharideGR-2aGR-2bGR-2CͶGPSȡ

ʲݵҶͪĵ߰-ˮ߰narcissin̻߰nicotiflorinܿ߰rutinƤ߰isoquerciTCMLIBinӢ߰asTCMLIBagalinuralenolڶuralenolneouralenoluraleninƤ-33-ѣquercetin-33-dimethyl ether-3-ѣuralenol-3-methyletherأuraleneƤأquercetinȡ߰uralenneoside

ʲݵĵϲַõݹأscopoletinâأȶκͪlupiwighteoneδͪأsigmoidinBԼʲgancaoninABCDELMNOPQRSTUV

2.ʲ ͸ʲأõʲᡢ18-βʲݴ⣬õ໯18α-ǻʲݴᣨ18α-hydroxyglycyrrhetic acid24-ǻʲݴᣨ24-hydroxyglycyrrhetic acid24-ǻ-11-ȥʲݴᣨ24-hydroxy-11-deoxyglycyrrhetic acid11-ȥʲݴᣨ11-deoxyglycyrrhetic acid3β-ǻչ-111318-ϩ-30-[3β-hydroxyolean-111318-dien-30-oic acidʲƴglycyrrhetolʲglabrolideʲݼisoglabrolideȥʲdeoxyglabrolide21α-ǻʲ21α-hydroxyisoglabrolideʲݻᣨliquoric acidȡֺͪɷ֣ʲ߰liquiritosideǸʲ߰ʲ߰ԪliquiritogeninǸʲ߰Ԫʲ߰isoliquiritosideʲ߰ʲ߰ԪisoliquiritogeninǸʲ߰Ԫ¸ʲ߰ʲ߰ʲ߰Ԫ-4-߰licurasidelicurazidʲ߰Ԫ-4-߰[neolicurasideisoliquiritigenin-4-apiofuranosyl1→2glucopyranoside]ʲglabraninʲݴglabrolʲݶglabridinʲͪglabroneʲأglabreene72-ǻ-34-Ǽ׶ͪglyzaglabrin7--2-׻ͪglazarin7--2-׻ͪ7-methyoxy-2-methylisoflavone7-ǻ-2-׻ͪ7-hydroxy-2-methylisoflavoneɻͪpinocembrinӣأprunetinâصȡֺʲ㶹liqcoumarinˮԶǼpectinʲݵĵϲַõ18β-ʲݴᣬ18α-ʲݴᣨ18α-glycyrrhetic acidᣨuralenic acidԼֻͪ໯ƤأƤ߰isoquerciTCMLIBinƤ-3-˫߰quercetin-3-glucobiosideɽͷӣkaempferolӢ߰أsaponaretinʲ߰Ԫʲ߰Ԫܾأgenkwaninɽͷ-3-˫߰kaempferol-3-glucobosideȡ9.7%ˮԶ1.6%

3.͹ʲ أʲݴ-3-ȩ߰apioglycyrrhizinʲݴ-3-ȩ߰araboglycyrrhizinƳɷУ18β-ʲݴᣬ11-ȥʲݴᣬʲ߰A3G2H2ȡֺͪɷ֣ʲ߰Ԫʲ߰ʲ߰Ԫʲ߰â߰47-ǻͪ47-dihydroxyflavoneʲݻͪlicoflavoneAʲ߰Ԫ-4-߰ʲ߰Ԫ-4-߰ʲöͪlicochalconeABCDëʲݲͪechinatinʲͪȡͪɷ֣5-ϩʲݶͪ5-prenyllicodione͹ʲݶͪglycyrdioneAB͹ʲglyinflaninABCD͹ʲݶͪA͹Aϵͬһʡֺβ-ȴβ-sitosterol

4.ëʲ ɷ֣ʲᣬʲȡֺͪɷ֣ʲ߰ʲ߰ëʲأglyasperinABCDأkumata keninƱʯȶأtopazolinʲͪlicoisoflavoneBʲͪsemilicoisoflavoneBʲͪlicoisoflavanone3-γγ-׻ϩάͪ[3γγ-dimethylallyl-kievitone]ʲʲ飨licoriisoflavanA1-1-methyoxyficifolinolֺ㶹ɷ֣ʲ㶹ʲ㶹isoglycycoumarinʲݷӣʲ㶹ͪˮԶǺ͹

5.Ƹʲ ͸ɷ֣ʲᣬʲ߰ABƸʲ߰glyeurysaponinֺͪɷ֣Ƹʲ߰glycyrosideâ߰ʲ߰߰߰ʲ߰Ԫ-4-߰ʲԪ-4-߰߰choerospondin㶹ͪ߰sophoraflavoneBĸѩ߰schaftosideɫͪ߰isovi-osanthinޣ̴-3-O-߰medicarpin-3-O-glucosideĵ߰򡣻β-޴β-sitosterolܲ߰daucosterolƤᣨphloretic acid

6.ϸʲ ɷ֡߰ˮõϸʲݴ߰Dglyyyunnanpro -sapogenin Dϸʲ߰ԪglyyunnansapogeninABCEFGHٸʲᣨmacedonic acidֺβ-޴ͪɷ֣ʲ߰Ԫ47-ǻͪ7--4-ǻͪ7-methoxy-4-hydroxyflavone7--4-ǻͪ7-methoxy-4-hydroxyflavonolݱ߰Ƹ߰yunganosideA1B1C1D1E2F2Ӻͩhypaphorine

7.ҹ۸ƷƷ⣬ԵΪδ֣ǵоҲ١Ҫ߼£1ʲݵĸ͸ʲᣬʲ߰ʲ߰ʲ߰Ԫʲ߰Ԫʲ㶹ʲ㶹ʲ㶹ͪʲݲͪAʲݻͪʲͪglycyrrhisoflavoneʲͪglycrrhisoflvanoneʲݻͪʲݶ-7-ѣʲʲͪʲFGHIʷӣ5-O-׷ӣ5-O-methylglycyrolصȡ

2½ʲݵĸ͸ʲᣬʲ߰ʲ߰ʲ߰Ԫ47-ǻͪâأʲݴëʹͪʲݲͪABʲͪBʲͪlicoisoflavanoneʲأʲݶȡ

3ʲݵĸ͸ʲᣬʲ߰ʲ߰ʲ߰Ԫʲ߰Ԫʲ㶹ʲ㶹ʲ㶹ͪʲ㶹ͪʲʲͪʲݷӣ5-O-׻ʲݷӵȡ

ҩá1.ϵͳã1.1.ãʲݵɷָʲض鰷ŽγɵĴʵԵıáݱʲԼٴϵµʣθҺ޼ٷơʵҲָʲݽȶԴţȮ鰷γɵáʲ߰Ԫʲ߰Ԫ͸ʲݸļ״ȡFml00ȶԶʵԵáʲݴŽĴõĿãָϸߡ

1.2.θڵӰ죺ʲθֱθᣬȮʵдܽθᡣFml00ʮָڸҩԼθŽĴƻθҺҩ߰ЭͬáFml00Եˡ鰷θҺá

1.3.θƽĽ⾷ãٴʹøʲͪ߰á峦ܳãʹ٣ŶȽͣȻ鰷峦ƽн⾷ãʲءʲݴƽá⣬ʲ狀͸ʲݴڷ಻ѡʲݼҺʲࡢFml00ʲءʲصȣҲ峦Եáܴھ״̬ʱԵĽ⾷á

1.4.ֱӸʲݼʲ7ͬĻͪ߰߰Ԫʵ֤н⾷Ϳ񲡵áԳȥʲصĸʲƼȡͪȻٴЧͼٸáӹʲݵļ״ȡзֵӡ

1.5.ãʲࣨ0.2ml/10gԤȸСθܽϢʹAAP200mg/kgipǻע䣩жСʣϢʹСԱáСϢʹ2-3СʱĸԪ½ЧӦǸλİ䶾ԴлءʲܶԿһЧӦ˵ıÿֵܲڶԴл¡ʲؿֹȻ̼жȱתøߣܼٸڸ֯ѧ۲ɼʲءʲݴƵĴ˾϶ᡣ֯ѧ۲ʾʲݴƵĴԭӣʲʲݴѪ̥򵰰׼Ҳڶ顣ʾֳɷ޽ԭܽյáȻ̼µĸ𺦶ģPʲءʲݴԸ𺦳ǿáʲݴỹǿȻ̼֬ʵɣCa2+ϸϸ𺦣ʾʲݴԸ𺦵ЧϣCa2+ϸڵúҪʲ߰ڻˮá

1.6.Ե֭ڵӰ죺ʲ䵨õĵ֭ڣʲ5mg/kgõĵ֭ڣýܺ󵨺á

1.7.ʲݽࡢۼ񲡵ٴЧ϶ЧɷֲǸʲµˮסѪѹߵȸöܵơ

2.ѪϵͳӰ죺2.1.ʧã˸ʲȡҺ1mlҩ1gͷշʧ2Ӻ1gkgע䣬ˮλɺԽɾʾdzԲ졣˸ʲԵĿͷշʧá˸ʲݼ࣬ʹӡʲض˷ãⶹȾԵĶԿãؾԵЭͬá

2.2.֬úͿãʲضʵԸߵ̴̴֢ߵĸѪѹ˾һĽѪе̴áʲÿ10mgkgע䣬5죬ʵԼø֬Ѫ֢ԵĽ֬ãѪ̴Ϊ89±4mg%ҩΪ43±4mg%ѪΪ168±10mg%ҩΪ90±4mg%Сĸʲأ2mg죩һʱʹʵԶӲõĵ̴ͣӲ̶ȼᣬ20mgֹ״Ӳķչʱ40mg죩ЧʲݴΣ10mgkgڷԸѪ֬ʵԶӲļнѪ̴֬׺β-֬׸ãڵĺʹڵĵ̴β-֬׺½ʲݴεĽѪ֬ͿӲý֮ۺ߰ǿʵ۲쵽ʲ1mMCP50AP5050%ѪƲλͬCisA-Teeˮϵͳпɼ35%ЧCisӶӰ˲ЧCH50APеC3Ľͣڲ巴Ӧʲƣص֢Ĺ̷Ӧ򻺽;ֹ֬ϵͳ͸ιܸƣ֢IJ̱ϡ

3.Ժϵͳãʲݽ͸ʲݺϼڷܸǷ׵ʲճĤ֢Ĵ̼ӶáʲݴԵãʲݴ˫οڷɴơʲݴᵨ501mgkg백ˮµ80%ĿԷЧɴlmgkgƤע޲졣ĸʲݴᣨ1250mg/kgʹСƣʲݴ5-ɫ֧ܾΣнıáԵ̼èµĿҲԵáʲͬˮƽʱ⻯ɵҲʾãӦʲݲͬҶԴ̼èĿЧΪʲ뿹޹ضͨġʲݻܴٽʺ֧ܵķڣʹ̵׿ȳ̵á

4.ϵͳӰ죺4.1.ãʲݾб̩ɻ⻯ɵĿã俹׳ɷΪʲغ͸ʲݴᡣʲݴԴѿףȩԽƤѿ֢Ⱦã俹ЧԼΪɵɻ⻯ɵɵ110ԴDz˽Խ׺ͿЧۣ⻯ɵΪ1ʲءʲݴֱΪ0.140.03ʲѿγɵãӳ͹֢ĵͽ˾طӦЧʲغ͸ʲݴᣬ֢ӦĢ񡢢򡢢ڶáСעʲ2550mg/kg컨ıƤӦʲݻƼͪСDz˽׺ϸͷŻѧáʲݿÿëϸѪܵͨ͸йأƤйأҲΪʲӰϸ̣ϸԴ̼ķӦԴӶ˿á

4.2.ãʲݴ1250mgkgСϵͳã򾲣ߣ½ͺͺƵȡ

4.3.ãʲݴ͸ʲطֱԷȵĴС󡢼þнáʲݴ40mg/kgǻע䣬Էȴã൱ˮ600mg/kgЧĴ޽á

4.4.ӹʲȡЧFm100ʹ⾷ãҩ߰Ҳ򾲡⾷ãߺԵЭͬã˵ҩʲɵĺԡ

5.Ƥʼã5.1.ƤʼãʲݽࡢʲؼʲݴԽ˼ֶﶼдٽơˮãƤʼȥƤͪƣӦÿˮ׼Ѫѹߣôȵİɭϲ

5.2.ƤʼãСʲأÿֻ100μgʲݴʹήӣƤʼƣ⻹пüõƤʼؿɵáôʱƤʼòԣֻƤʼãûйءΪûƿƤʼƻ뵰ʵĽϣʹѪƤʼ࣬ӶǿԡƤʼ봹ǰҶķӦڽǿѪкߴһ̶ȺֹͣƤʼܴӰСƷȸʲдƼػԣδڷӹѳӰ첻

6.ֳϵͳӰ죺ʲἰΣעǿãԴӰ졣Ƽʵ԰׽ʯγɡƴƼضԳ궯ӹãгٻѳͬáʲضԴпãų٣ųҲȼ١гٵĴ󣬸ʲʹƺͼصų٣˵ͨƤʼʵֵġʲݴἰҲԵĿáΪʲǿСܶƺȵնֿã÷ʽȥƤͪͬǶСֱܵá

7.߹ܵӰ죺7.1.ãӸʲȡһָ壨LxеʡᡢǼʲᡣ󾭾עùBPOѪ׵ףHAS󣬾ֹݿ֢״5ݿ˷ʺʾΪ100%󾭸LxȻпԭLxСĹӦΪ25%Ϊ21%Lxݿ˾Եıã󱣻ǿLxСѪ忹ùЧΪ4-16δѪ忹壬鿹ЧΪ256Lx鰷ĺϳɣǿСעѵ׿ԭǰ3СLx 0.2mlǻע䣬15죬ֱⶨѪIgEIgGͷ鰷LxСݿԵıЧӦƿ

7.2.Էdz߹ܵӰ죺Сڸʲ75mgkgǻע䣬ÿ1Σ4죬ĩθҩ󣬸ӡī֭ȡѪָKֵʲKֵΪ0.048±0.020Ϊ0.029±0.015ȽŲ죨P<0.01ʲСԾעָ̼ʾǿ״ƤϵͳĻԡʲ˸ʲͬá

7.3.߹ܵӰ죺⿹ϵͳо˸ʲԶ¡Ӱ졣һŨȵĸʲʹӡ⣬ĩѪϸճϸӸŨȸʲ󣬽Һм뵥ϸֶ̽PWM̼յӰ졣⿹ǿⶨҺаϸ1IL-1ʱ֤ϸ1ࡣʾ⿹ǿϸ1ǿйءСǻעʲᣬͬʱעѪSRBCߣԭע4ƢϸѪĿհγϸԼ30mgkgĸʲʹǿıҩʱ뾲עѪʱͬʱԭǰlʲᣬɴٽԭע2ʲǿɴ˿ΪʲҲǿʲݻԴٽ򵰰AƢܰϸDNA͵ʵٽDNAϳɵŨΪ100μg/mlDNAϳɸ߷48Сʱ԰׽-2IL-2ҲԵǿáDNAʵϳɼ׽-2ӰƽеġţѪ׵ףBSAΪԭһԼѪ岡ģ͡ʵ鶯ţѪ׵׺357918β-ʲݴ200mgkgע䡣ʵ鶯ѪпţѪ׵-IgGţѪ׵ߺ6l2߷壬ʵԸڶ顣鶯ѭţѪ׵-ţѪ׵׸ǰȽϾߣŲ𣬵ͳѧ졣Ѫвֵʵ϶ߡ18β-ʲݴԿѭ߸γδӰ졣ʲؿߵ򵰰AյƢϸγ-أγ-IFNˮƽPHAĸˮƽӰ죬ӵ򵰰AյزŨΪ200ug/mlʱΪ48СʱϸŨ1×107mlΪˡĸγΪʲ100mgkg·죩×7,θС󣬾ⶨƷκǰE2ǰF2αĺϳɡʲᵥСθl00mgkg·죩120 LD505ƢǰE2ͻգcAMPӣܰϸ8×10-10molL8×10-7molLĸʲݵŨ£ǰEҲӣǸʲߵڵ;֮һͨͷ鷨ⶨʲ臨BALBCСȻɱϸNKϸʱ1×10-7-1×10-1mg/mlŨʱСȻɱϸԾǿԻ߹ܾҪá

8.ã8.1.̲ãʲܹ߰ƻԹܵİ̲ϸHIV0.5mgmlĸʲ߰԰ֳ̲98%ϣ50%հγֵΪ0.125mgmlڸʲ߰ư̲ת¼øʾָͨTϸáʲе¶ڵŨʱʲȣʾ԰̲ϸֳЧ

8.2.ãʲݶǾԵĿˮԿײٲ3͡1͡ţȻԣϸķʹ֯ϸõȾǰ24СʱҩˮԿײٲ3壨P<0.01Ⱦҩ4ֲãP<0.01ҩҺ벡ҺͬʱϸҩҺ벡Һ372Сʱϸ㣬4ֲҲáûƿǶ෽ģҪֱáʲԵʲضԹˮ-״áʲݴ8mMŨ37洦͵15ӣȾ۴107102ЧʲݴƺԵáʲضȺˮ-״VZVȾ̥άŨΪ0.55mgmlŨȶԳάϸȫûжԡ2mgmlʲؿʹ99%ˮ-״ʧŨȵ0.08mgmlʱҲʹˮ-״ʧ

9.ãʲݵĴȡPʲݴԽɫ˸˾󳦸˾װԭ漰γãѪڵ£־ɱװԭʲݴԵγЧŨΪ30-60μg/ml

10.ⶾãʲݽ༰ʲضijЩҩжʳжڴлжһĽⶾⶾõЧɷΪʲأⶾΪʲضԶãʲˮȩ붾ϣԼʲƤʼǿĽⶾȶ෽ۺõĽʲܶԿСʿжСעʿ0.1mg10ڶΪ100%ʵ飨Ԥעʲ12.5mgΪ58%ʿΪ0.03mgΪ58.3%ʵʲݽ͸ʲضнⶾãˮȩʿ̹ͿɿнԵĽⶾãӡѶءëܿͰͱ׵Ľⶾô֮ãSulfonalƷ޽ⶾãصжмǿ򡣽ⶾõijɷΪʲءбо˸ʲݼɷֶ鰷жӰ죬֤ʲάB1ϵĻⶾǿʲشֽ֮ȩĽⶾϲʲض˷綾нⶾã԰׺Ҳнⶾá֮ʲݼƼҩжʳжڴлжϸصȣһĽⶾáʲݿʹСȽϸɫP-450ӣԸҩøյãʲݽⶾĻ֮һ

11.ãʲԴˮΰСϸˮEACϸ̬ܲѧϵı仯Ƥֲļ䵥ζСϸˮãڷҲЧʲݴԴֲOberling GuerinãܼʱСϸˮEAC-45ϸ΢áʲ߰ԴˮΰСϸˮϸ̬ܲѧϱ仯ƴ(12-0-teTCMLIBade-canoylphorbol-13-acetate,TPA)Զױ죨DMBAСƤĴٷãɱʲơ

12.ã;ӼԶ΢λ񾭸϶λΪ͹ָ꣬о˸ʲݴڶܵӰ졣עʲݴ100mgkgɶĶ΢λ񾭶λ񾭶λӦֵͣʲݴڶܵáʲ͸ʲݴʹŨΪ8×10-3mg/ml4×10-2mg/mlʱøԵá50%ʱҩŨȷֱΪ25.6±1.4μg/ml21.8±1.1μg/mlҩЧɷֶ֬øʾһǾͻơ

ԡ1.ʲݶͣ糤ڷãˮ׺ѪѹߡʲˮСLD50עΪ1.9432±0.467gkgǻעΪ6.8466gkgƤעΪ7.8192gkgʲݽСƤעLD100Ϊ3.6gkgΪԣʲСƤעΪ1gkgʲݴСƤעLD50Ϊ308mgkgFml00СǻעLD50Ϊ760mgkgʲݴС1θǻעLD50Ϊ101mgkg

2.øʲˮθ6ܣÿ2gkg۲Զԡҩ鶯رȶӣδָףʵڼнͣΪڸҩڸʲݵƤʼ¡ʲݴ״ٹܣнͻлơ

ɫףȡƷĩ1g50%Ҵ25mlȡҺҺȡʲΡʲ߰ʲ߰ƷƳɶƷҺȡҺֱͬһ轺G-CMC ϣ-Ҵ-ˮ512չ50%Һ濾ҺɫƷɫӦλͬɫɫߡȡƷĩ1g40mlˮԡϼȻ1h˹ҩӼ״30mlˮԡϼȻlh˹Һɣˮ40mlʹܽ⣬ˮҺȡ3Σÿ20mlϲҺˮϴ3ΣˮԡɣӼ״5mlʹܽ⣬ΪƷҺȡʲ臨ƷӼ״Ƴÿ1ml2mgҺΪƷҺȡ2Һ1-2μlֱͬһlҺƱĹ轺GϣԴ---ˮ30224Ϊչչȡɣ10ҴҺ105ɫƣ365nm¼ӣƷɫƷɫӦλϣͬijȻɫӫߵ㡣

ơ1.ʲݣȥʣϴˮ˳͸ʱ̳͸Ƭɡ

2.˸ʲݣȡʲƬķ뿪ˮȣƣùĻΪɫճΪȣȡÿʲƬ10025-303.׹ۡʹʲݣȥͷβ⴦þƽɣϸʹһͿˣ־Ϊȡ

4.Ŀ˸ʲݽóˮպʪ֮ȥƤýˮ졣

5.䱾ݡװ賴á

ζʣƽ

龭Ƣθģξ

ΡУֹʹֹȣкⶾҩ뵡ʳ٣ƣļ̣ʹ磻֫μʹꣻʺʹӸʹС̥ҩʳж

÷ڷ2-6gҩСΪҩԴ󣬿10gңжȣ30-60g벹ҩãкҩáãˮϴգĩ

ע⡿1.ҽѧšá

2.ҩƷ塷ζ̫𣬲ҩв˶ã˼ʳҲ

3.ӣͣ룬仺

˸ʲ˺ۡ顣ʲк˺ۡ¶Ƣ顣ʲݸ˺ۡӪѪˡҩʲ˺ۡ֫ʹ߸ʲ˺ۡθ㣬ˮͣн֤

1.꽣ʲݣ߲֮ԸʣܳȣƽƢθ㣬кĻ֮£ԪɢаȣȥʹѪĻƢмʹƤߣ˱ܻ֮Эҩʹ֮ҩ֮ȣҩ֮亮ߣ֮ƽ

2.ҺݡøʲݣҲθøʲݣҲҩ֮ǺҲԻҲСв֮˲Ρ֮£øʲߣе֮⡣вøΪ֮øΪ֮йҲ赤֮ʣԪʲ֮ҲƣԸʲ֮Ըк֮Ըʻ֮ܰͲʯҲڴ˿ɼ֮⡣ζ֮ãֱжйжɢֹᣬжΡݡǸ֮ζϿ£ڿ⣬клвй֮ӡ

3.岹šʲζʣ½ҩãִ󻺲ֱ

4.ݻԡʲݣⶾ֮ҩҲƢθ֮ЭͲ֮ӪˣƢԪ㣬˥飬ƽЧΡβҲʺʹʵճοʣ̵ѿԣӡ£̵˳Ρ磬˶߱ΪѪߣβ֮ûˡҩֹʹýϻضһ۶ô̥̣֮ɢӸڴ֮ʡôȴ֮ҩøʲԻƣ֮ҩøʲԺԡԪƣʵɸʲݹӣ治£ͶΪʲ߼ͨ

5.ͨʲݣƽ֮ƷƢθʱԻͨʮߣҲʣ֮ζʸʲΪй¼ƣȨƣƢòڽҲƢ̫ߣתԻƢʳʣУΪʵҲײʵÿʲݣβ˼֮Ү6.ʲݣζʣк֮ԣе֮ʶҩ֮䶾ҩ֮ԣҩ֮⣬ҩ֮١Ργ֪֮Ҳ֮Σ˭ɡаȣǣƢθ⡣ҩѪҩѪɣʳƹϡΩӣͣ룬仺ɲ֪Ҳ

7.ҩƷ塷ʲݣкɢаӸףʹҩθȣȥʹ˸֮Ҳ¶УƢ黬кθڿʣȿԣ룬𣬴˸Ƣ֮Ҳζ̫𣬲ҩв˶ã˼ʳҲ

8.ݱҪʲݣǢ̵񱣬ʮӸʲݣԫνˣ뺣ͬãϪ񩣬ܾͬУپиʲʲҩʲ˸ʲԼ֦ơС桢θС׻޲øʲݣɹСк𡢽ⶾиʲΪãܽЧ˹ŷҲκʱʦÿøʲݲֶֹ֪ʼԺˣϰɷ磬βƣڴˣʧ

9.֤˺ۡҪԡУΪʮøʲߣٶʮǸʲ֮࣬غϸʲݣʼҲҩ֮ɢߣڣơ֦ߣ¶ϣθʳƸʲݵߣ妣桢ǵߣͣ׻ҶʯߣڶȺк÷Բȣߣƣ÷guȣ޸ʲݵ䣬Ϊս֮ƣƸۣʤɲʤڪϾʤ֮Ү֮ΪˣѪֹȺϣΪŧԻף֮׶δŧҷDzҲǧ𷽡ν𴴶ϵѪֹ߲öۻơʯҡݻҵظʲݡΩҪԡɢСyϸҶɣϸʮ֣ʲݶʮ˷֣Ը٣ȡ⣬롶ÂӡʲڽߣDZĻ𼱼֮δϣѪСȺϣսй׶ŧŧӸңᣬӸߡʷɣƤ֮ϴ֮ͨѪߣҩоӪֱؾ֮Ըʲ֮ʻⶾкСdz֮Ի֮Իʵй֮Ϊӡʲ֮ˣȷвͬʳаν𴴡ⶾáв顢ֹʣãϢ֮ӡ

10.塷ʲݴʣ书ֹڲҲָܻ֮йصøʲԼ֮֮ңصøʲ֮ߴߵ֮棬ߵ֮ھԻ֮ҲȻؽͽֱ׷ֲ֮ɼʲݣԸ֮㣬֮ս˽ݣͶҲԻ߼ɸʣŻҼɸʣƼɸʣ֤֮˸ʲݣ˶֪֮ӣȻδ壬Լʪ̵֤Բܽ壬øʲݣƣʼŻΩ̫ʶְʲδʼд˵ϡĿԻʲͷӸף·ңԸʲݽӸ׶Ǽӽⶾһ֮ȻӸ֮ʪڳ㣬֤֮غʪΪʲݸɡŶͶ֮ʳδȼ亦

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