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2017-08-15 10:38 Ķ:

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ƴJio G Lán

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Դ«ƽʹֲʹGynostemma pentaphyllum Thunb.Makino״ҩ^ɼϴɹɣзۡ

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ƴJio G Lán

ӢHerba Gynostemmatis Pentaphylli

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ԴΪ«ֲʹGynostemma pentaphyllum Thunb.Mak.ȫ^գɹɡ

ԭ̬Ԯݱϸëë볣2ѻ򲻷ѡҶ״57СҶɣСҶƬԲ״ΣСҶмСҶƬ39cm1.53cmԵоݣҶж̸ëëԲ׶򣻻СֱԼ3mmƬΣԼ0.5mmƬΣԼ2mmΣʱɫ78£910¡

ֲɽʪڰա㽭㶫ݡָضࡣ

ѧɷ֡ʹ߰aypenoside15234812ֱ˲߰Rb1Rb3RbRf2ṹͬлͪࡣ

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ҩơʹ

ƴJio G Lán

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ʹʼڡȻıݡƣ"ʹҰУҶСҶСϱоݣݣҶ̣ҶһСɫп׻ߣ㶹ɫϺɫҶζ"̬֡

ԴҩĻԴΪ«ֲʹȫݡ

ֲﶯGynostemma pentaphyllum(Thunb.)Makino

պʹأÿġɲ3-4Σϴɹɡ

ԭ̬ʹ Եݱϸ֦͹ۣë豻ëҶҶ3-7cmϸ2磬ϡһëëҶƬĤʻֽʣ״5-9СҶͨ5-7״ԲλԲ״ΣСҶ3-12cm1.5-4cmСҶСȶ˼̽⣬Ե߲״ݻԲ״ݣɫ浭ԣԵ߲״ݻԲ״ݣԣ浭ɫӲë6-8ԣƽ̹ͻϸ״꣬ۻΪԲ׶򣬻ϸ֦10-15-20cm֦չ3-4-15cmʱСҶë˿״1-4mm״СƬͲ̣5ѣƬΣڵԣ5ѣƬ״Σ2.5-3mmԼ1mm1ԵԵë״Сݣ5ϳƻΪԲ׶򣬽ۻСࡢھۻӷΣ3̶ֲ棬ͷ2ѣ߶С˻5ʵΣ5-6mmΪɫ⻬ëں2ţ״ΣԼ4mmҺɫɫ˶ۣΣѹ״ͻ״ͻ𡣻3-11£4-12¡

ֲ̬ں100-3200ɽСɽ»С

Դֲֲͳϸء

ࡿѧ ϲůϲʪֱ䣬ͺԲҪϸѡɽ»ɽֲԷ֡ɵɰΪá

༼ ӡֶκ;Ǥ己ֳӷֱֳԷֱ3-4£о40cm1cm15cmʱ15-20m硢硣Էֵ紲糤2-3ƬҶʱ40cm×mԽֶηֳ3-4£ڳɳԼ5cmСΣÿ1-2ڣ50cm×30cmѨÿѨ1СΣԼ3cm1hm245000ңԺʱˮʪǤ己ֳ5-7£ѵϾ³ɶΣÿα3-4ڣȥ2СҶ10cm×10cmб紲ˮʪ¸ѿ10-15cmʱ30m×15cmѨֲ

עݣڽˮܷ硣һɴ2˲֧ܣֲܣԵ׷1Σʢ׷ʩϷ2-3Σÿÿ1hm2ʩ105-180gÿո׷1Ρƽʱע⽽ˮʪɺ꼾עˮԡ

״״ ƷΪȫݣϸɫɫݹƣϡëףʪչҶΪҶСҶĤʣͨ5-7ö9öҶ2-4cmëСҶ״ԲλԲ״Σ1öϴ󣬳4-12cm1-3.5cmȶ˽⣬ШΣ汻ëҶԵоݣݼâɼʵԲΣֱԼ5mm3-5mmζ߲࣬

΢ Ҷ棺Ҷ±Ƥ1㳤ϸɣⱻʲ㡣Ҷ֯ͣդ֯1-2ϸɣͨ֯3-4ϸɡ±Ƥͻڲ2-3ϸά͡ Ҷ棺ϱƤܱڽƽֱ±Ƥܱ΢״Ϊʽ±Ƥзëëë5-14ϸɣԵ״ƣ120-360mm

ʶΣƤ1бƽϸɣڽϸͶϸë紦к֯4-6ϸɣƤڷΧƤԵİάڷ9-10Сȵ˫άУƤ߼ʯϸȺ貿ϸںֱ12-28mmĵ

ѧɷ֡1ʹ߰(gynosaponin)TN-1TN-2ʹ߰(gypenoside)I→LXXIX79Т󡢢ṹ˲߰(gensenoside)-Rb1Rb3RdF2ͬ6"-˲߰(6"-malonylgensenoside)-Rb1Rd6"-ʹ߰V(6"-malonylgypenoside V)ȡЩ߰߰ԪУ˲ζ(panaxadiol)2α-ǻ˲ζ(2α-hydroxy-panaxadiol)(20R25S)-12β25--2026--3β-(20R25S)-12β25-epoxy-2026-cyclodammaran-3β-olݣ(20R25S)-12β25--2026--2α3β-(20R25S)-12β, 25-epoxy-2026-cyclodammaran-2α3β-diolݣʹ߰Ԫ(gynogenin)(20R)-2124--3β25-ǻ-23(24)-ϩ-21-ͪ(20R)-2124-cyclo-3β25-dihydroxydammar-23(24)-en-21-oneݡ

2ֵõ4µĴ߰ǣ(20S)3β2023ζ-ǻ-24-ϩ-21--2123--3-O-β-D-ǻ(1→2)-α-L-૰ǻ-20-O-β-D-߰(20S)3β2023-trihydroxydammar-24-en-21-oic acid-2123-lactone-3-O-β-D-glucopyranosyl(1→2)-α-L-α-L-arabino-pyranosyl-20-O-β-D-rhamnopyranosideԼ(20R)칹(epimer)(20S)-23ζ-ϩ-3β202526-Ĵ-3-O-β-D-ѻ-(l→2)-α-L-૰ǻ-20-O-β-D-ǻ-26-O-߰(20S)-dammar-23-ene-3β202526-tetraol-3-O-β-D-glucopyranosyl(1→2)-α-L-arabinopyranosyl-20-O-β-D-rhamnopyranosyl-26-O-glucopyranosideݣ(20R)-25-ϩ-3β202124ζ-Ĵ-3-O-β-D-ǻ-(1→2)-α-L-૰ǻ-21-O-β-D-ǻ-24-O-߰(20R)-dammar-25-en-3β202124-ζ-tetraol-3-O-β-D-glucopy-ranosyl-(1→2)-α-L-arabinopyranosyl-21-O-β-D-glucopyranosyl-24-O-rhamnopyranosideݡ

3޴ɷ֣524-«ϩ(cucurbita-524-dienol)2424-׻-5α--8-ϩ-3β-(2424-dimethyl-5α-cholest-8-en-3β-ol)(24R)-5α--7-ϩ-22-Ȳ-3β-(24R)-5α-stigmast-7-en-22-yn-3β-olݣ2424-׻-5α--7-ϩ-22-Ȳ-3β-(2424-dimethyl-5α-cholest-7-en-22-yn-3β-ol)2424-׻-5α--7, 25-ϩ-22-Ȳ-3β-(2424-dimethyl-5α-cholesta-725-dien-22-yn-3β-ol)޴(spi-nasterol)α-޴(α-spinasterolchondrillaierol)2424-׻-5α--7-ϩ-3β--(2424-dimethyl-5α-cholest-7-en-3β-ol)(22E)-2424-׻-5α--722-ϩ-3β-(22E)-2424-dimethyl-5α-cholesta-722-dien-3β-olݣ2424-׻-5α--725-ϩ-3β-(2424-dimethyl-5α-cholesta-725-dien-3β-ol)14α-׻-5α--9(11)24(28)-ϩ-3β-14α-methyl-5α-ergosta-9(11)24(28)-dien-3β-olݣ2424-׻-5α--3β-(2424-dimethyl-5α-cholestan-3β-ol)24α-һ-5α--3β-(24α-ethyl-5α-cholestan-3β-ol), 14α-׻-5α--9(11)-ϩ-3β-14α-methyl-5α-ergost-9(11)-en-3β-olݵ(24R)(24S)IJ칹壬4α14α-׻-5α--79(11)24(28)-ϩ-3β-4α14α-dimethyl-5α-ergosta-79(11)24(28)-trien-3β-olݣ޴(isofucosterol)β-޴(β-sitosterol)ȡ

4ֺͪɷ֣ܿ߰(rutin)½߰(om-buoside)½(ombuin) (malonic acid)άC(vitamin C)춬(aspartic acid)հ(threonice)˿(serine)Ȱ(glutamic acid)17ְп̡ͭ18Ԫءζɷ֣Ҷ(phyllodulcin)

ҩá1߹ܵӰ죺1.1.ԷߵӰ죺Сʹ10g/kg30g/kg10գƢȶӣԴٽ˾ϸϵͳѪн̼ʣߵϸɹܣ30g/kgʹӡбСʹ߰GPs50mgkg10գƢس˫ãСλƢأmg/10gأʹ֮ӣλƢʹ֮Сmg/10gأСλӰ죬λʹ֮ԼССGPs200mg/kg400mgkg10գԶԿٺƢᡣСʹˮ崼ˮ0.5g/kg1gkg2gkg5գ1.0g/kg2.0g/kgʱƢءСGPs400mg/kg5գԻ١ƢͳϵĤܰͽļ׿ãӰ졣бСGPs300mg/kg7գءƢӰ죬żǿǻϸɹܡα-շСǼ˥ģͣ3%ʹࣨҩ150%Һ50գʹߵİϸܰϸİٷʽӽ飻ʹ͵ϸٷӽ飻ģήƢӣҩָָ飬ƢָҲָģѪܾøŽͣʾ忹Ⱦ½ҩܾøӲ顣СGPs60mg/kg100mg/kg7տѪϸǿɽĸʱĻѧֵͬʱֵָϸҲߣԴշƵСGPs80mg/kg120mg/kg7գϸֵͷָ߸ΪţʾGPsϸʹһЩϸµĶϸǿܵµİϸɹܡбڷʹ20gkg15գԷξϸɹá

12ܰϸתͰ׽-2IL-2ڵӰ죺ʹˮ崼ˮ10μml100μg/ml1000μg/mlʱߵ򵰰AConA֬ǣLPSյСƢܰϸֳӦGPs10-200μg/mlܴ̼СƢܰϸԷ[3H]ऺգH3-TdR룬ConA飬Э̼ͬСƢܰϸ룻⣬ܴ̼ƢܰϸԷIL-2бGPsС2.5-20μg/mlٽСƢTBܰϸתDNAøԣٽܰϸDNAϳɣGPs(40g/ml)෴ãGPs40μg/ml80μg/mlConALPSֲѪأPHAշСƢϸֳӦԴٽãҲǿƢϸIL-2ķڡСƤעGPs10mg/30mg/kg7죬ConALPSյƢTBܰϸֳӦǿã10mgkgøţƢϸIL-230mgkgøԡԻ߹ܵСƤעGPs10mg/kg20mg/kg7죬ָߣIL-2߳磬TϸֳӦǿֽ20mg/kgøšܵĿGPs߹ܵСøšǻעGPs10mg/kg20mg/kg7죬ƢϸConAյֳӦǿ10mgkgΪšͬʱԸЧҺɫ׼ⵥ񾭵ʣȥأNE½ƢԽͣƢϸֳӦʸأ⣬ѪƤͪˮƽ½ʾGPs߷ӦӰ죬ڷڵ磬ǿþйϵGPs10-80μg/mlЭͬConAʹСƢϸ֮IL-2ߣСǻעGPs100-200μg/kg8죬ЭͬCondʹƢϸIL-2ߣշСƢģͣIL-2ԱСԽͣGPs⡢ھЭͬConAʹµIL-2ߣָӽˮƽ⣬GPs10μg/mlPHAշϸֳӦǿãŨӻ򽵵ãΪGPs˫ãܰϸPHAշֳӦԽܰϸͣܰϸPHAֳӦϵʱGPsǿãܰϸPHAֳӦǿʱGPsΪáijЩƺʹÿ˲40mgÿ2ÿ3Σ1£ܰϸת(3H-TdR)żǿãIgGIgMԼ١

13Һ߹ܵӰ죺Сʹ10g/kg50g/kg8죬ѪжϸSRBCԿѪصĺСʹˮ崼ˮ0.5gkg1.0gkg2.0g/kg5죬SRBCСܴٽƢ࿹ϸPFCγɣ2g/kgܴٽѪصγɡСGPs100μg/kg10죬SRBCСѪˮƽ˫ãԻѪˮƽ½ԶԿãδָˮƽбСGPs400mgkg5죬SRBCշѪӰ죬Ժ180Сշ߹ܵ´SRBCѪغPFC٣GPsŵıáСGPs300mg/kg7죬ѪIgGһʹGPsʹIgG1gMߡбСʹ29g/kg25죬ɽƢ࿹γϸɽСѪSRBCζȡ

14ϸ߹ܵӰ죺Сʹ10g/kg30g/kg12죬ԶȱDNCBTϸ鵼ijٷƤӦǿáСθGPs200mg/kg400mg/kg10죬ԻE-õ廨γʵĽԶԿãʹָˮƽGPs50mgkg10죬СE-õ廨γʳ˫á180СGPg100mg/kg12죬շ߹ܵ´GPs150mg/kg300mg/kg15죬SRBCߵƢϸõ廨γϸļԵıáα-ɵǼ˥ģС3%ʹҺ0.5ml/50죬ʹѪ͵ܰϸα-øANAEߣҲʹСDNCBƤٷͳӦǿСGPs300mg/kg7죬ļֲ췴ӦδӰ졣ӦMcAb-A-Eõ廨飨ֱӷԷʪಡزಡѭ˵ѪϸоӦѭʱTϸȺCD3+CD4+ϸͣCD8+ϸߣB+ϸҲͣ켫š⾭GPsú󣬽͵CD3+CD4+ϸB+ϸߣߵCD8+ϸԽͣʾGP8пӦãӦʱеá

15ȻɱϸNKӰ죺GPs100μg/ml200μg/mlʱѪNKϸмǿã400μg/mlʱáСθGPs400mg/kg12죬ԻƢNKϸԽ׿á

2ã2.1.СGPs50mg/kg7죬СS180С40%Сʹ1g/kg2g/kg10-12죬S180֯ȽСҸµãʷֱ28.9%38.1%S180ֲý7.5g/kgʱáԸǻְѪL615С󣬹2g/kg7,ȽӳΪ37.4%P0.05Բ鷨ӳʿɴ66.6%P0.01Ӽ7.5g/kgʱӳʲӣС29.6%1-2g/kgСֲԸΰáǻS180СθGPs3mg7죬ʹÿ1mlˮϸԵڶ飬ڷеϸҲԼ٣ҩ鴦DNAϳǰڣG1ڣϸİٷԸڶ飬DNAϳɺڣSڣϸٷԵڶ顣ʾGPsϸDNAϳɡGPsS180СƢϵϵӰ죬ѪϸTHϸBϸIgMIgGԸڶ顣ʾGPsϸ߹ܣҲǿҺߡ

2.2.GPsĿҪǡļСGPs10죬30mg/kgʱS180ʴ87.1%300mg/kgʱֻ61.1%600mg/kgʱʽ21.1%⣬GPsS180ϸͬ5СʱȡȾɫȾɺɫΪϸGPsŨΪ0.38%ʱȾ54%ŨΪ0.55%ʱȾΪ82.7%ŨΪ0.75%ʱȾΪ87.5%ʾGPsS180ϸҲֱɱáϸˮСֱʹϲּNGP 1g/mlʽʹ֯ˮˣFGP1g/ml0.3ml󣬸ˮ͹15죬ʵ͹30죬ԸˮСNGPFGPʹӳʷֱ40.7%46.6%ʵСʷֱ33.1%36.2%ϸˮСѪԴøANAEϸʣֲѪأPHAķתʾСͣFGANGPСָ꣬Ӧ5-ऻİϰСƢܰϸANAEʣPHAķתԼNKϸ԰ϰϸĶԾͣFGPNGPС󣬶ָߡ

2.3.ʹˮ崼ȡ5mgҩmlʹΰϸSMMC-7721ϸԲĤ״öһDEN´ΰǰģͣԺGPsϣGPsŨ1.5gkg0.48g/kg14죬DEN¸ΰķáGPs1-10μg/mlʱԷΰϸA549Calu15929ǿڹHela S3᳦ٰColo205ͬŨGPs˻ܰϸãȴдٽϸֳáʹȡҺ1.0gҩml߰6.44mg/ml10.697mg/ml1-20μlmlʱ˴󳦰ϸHce8693DNAϳãأڽϸŨʱάϸãŨȵ10μlmlʱԴ󳦰ϸãάϸ޲Ӱ졣ڴ͹ǼϵC20C21ǻ߰ķΰӹɫϸЧϸֳ޲Ӱ졣

3ӻ˥ãʹӳϸĴƤϸGPs200μg/mlҺʹϸ27ܴ22̥ζάϸҲƽ鴫51GPsɴ590.5%1.0%ʹˮҺ𻯼ӬãʹۼӬʱ䡢ƽӳã1%ʹ黹ʹӬڳ绯øSODߣȩMDAŽ͡0.5%1%ʹĹӬ۹Ӭƽֱӳ11.8%12.2%0.25%ŨȶԴӬƽֱӳ18.5%24.1%0.5%ʹڷڵѿʼҩƽȳɳ棨30d䣩ʼҩӳӳٷǰΪ53.3%ԣ46.1%ԣΪ14.4%ԣ9.7%ԣʹȡܴٽӬ׳ԹӬԻδӰ졣5Сúʹϣ2.5gϺ0.1gҩ4£50%, 0%ι2¼СSODԡd-շСǼ˥ģͣͬʱÿոǻעʹҺ15mg/󣬹40죬ŶԿ˥ģСѧϰӱܷӦ½ڵøMAO-B쳣߼֬ʵʹ˥ģСήٻָˮƽƢҲָˮƽСιʳʹˮɽ200mg/kg3£ʹԡ֯֬ʺ٣֬ʺŲ죬Ҳȶ͡ιǺ0.5%0.25%ʹˮɽ2.5£ġΡ֯֬ʣLPOԽã0.5%ŨѪ͸̴ܵͶԽá⣬250μg/1.5ml500μg/1.5mlŨʱԴġԡ֯LPOŽáСGPs80mg/kg2£ѪκLPOŽͣҿ߸κSODԣɽСƤǸẬʾGPsпӻ˥á

4֬ʴлӰ죺4.1.ȡǸ֬շ֬Ѫ֢Ѫ֬ߣѪLPOGPs500mg/kg100mg/kgзã7ڣѪ̴֬ܵˮƽ½LPOơʵԸ֬Ѫ֢ùGPsl00mgkg4ڣԶԿѪ̴ܵTCߣͣTG͸ܶ֬׵̴HDLcӰƺHDLпӲAs֮ƣHDLcTCˮƽ߶ӣֵȽ岻ĿǰAsIӲָ(TC-HDLc)HDLcݻHDLcTCAsԣGPsԽAsIHDLcTCɶԤAsл塣As߿е̴Ҫܶ֬ףLDLʽڶڣշܱƽϸѪƤϸGPsԽLDLˮƽԤAsá

4.2.֬Ѫ֢ѪLPOļ֬ʣLfԸãGPsԽѪLPOļLfGPsпɻѪĤá֬Ѫ֢GPsl00mg/kg300mg/kg15죬ʹѪTCTGLDLͣHDL/LDCֵߡСˮʽGPs300mg/kg4£ƻ򣨺ͣļLfγɡǻעʵҺĸ֬Ѫ֢С󣬹GPs200mg/kg7, ԽѪTCŨȣ֬ϲĸ֬Ѫ֢󣬹GPs200mg/kg7죬ŽTCLDLͼܶ֬ףVLDLHDLHDL/LDLֵ

4.3.֬Ѫ֢߷GPsɽѪTCTGLDLcб֬Ѫ֢߷GPs40mgÿ3Σ30죬ԽѪ֬ףapoB100֬ףLPaĺΪȫ֯ĹѪֹӲҪ壬ѪHDLcLDLcVLDLcTCTGĺӰ졣Сýʹ13죬߸SODͬʱҲǿSODȡܣ˱SODá⣬GPsȽLPOãθGPs0.25g/kg 8죬ڳУ˵ɻģͣҩ10죬ʹѪLPOԵڶգϸSODҲԸڶ顣26䣩ˮGPs 0.05g/kg 80죬ҲԽ͸LPO,ǿϸSOD

4.4.⣬GPs 2.5-160μg/mlʱFe2+ -װᡢά C-NADPHȻ̼շĴ΢MDAԼԷMDAɾãҳЧϵȼȽϣGPsЧԸ˲߰Fe2+ -װʹ΢塢Ĥ΢ճ󣬱ʾĤԽͣͬʱ GPs2.5-160μg/mlɶԿĤԵ½δ˵΢塢ĤӰ졣û-øX-XODϵͳշͷɻˣX-XOD£ͷߺ󽵵ͣܲӦ̣˷ߣշӣļSODԽͣMDAߡȸGPs50μgmlٸX-XODͷ߷Ƴ٣为ԼãͬʱתX-XODµͷӦڡ˷Եĸı䣬ļ֯MDAԻָʾGPsļбáKrebҺɻOFRʹAchշѪܻŰٷԼͣGPs25μg/ml50μg/ml100μg/mlɱѪƤOFRˣGPs50μg/mlܱINDOϣX-XODҲʹѪܻŹܽͣGPs50μg/mlɶԿX-XODáMbҲѪܻŹܡGPs100μg/mlҲܶԿMbáѪƤͷǰлأPGI2ƤɳӣEDRFȵѪǿ

4.5.OFRѪƤͷEDRFйأGPsܶԿMbEDRFѪŹܽͣʾGPsܱEDRFԡGPsÿɱINDOϣӱGPsÿͨٽ֯ͷPGI2Ĥ֬ʹйء

4.6.GPsɼ԰ϸڳӺ͹⺬˵ϸϸGPsɼٽĸǴĻѧFe2+ -װᡢѪ-NADPHڸ΢ѪƤϸLPOӣGPsһã˶˵ĸ΢ĤԣGPsתѪƤϸøԣЩϸøй©ʾGPsɱĤˣGPsЩ㷺ĿЧԶּ綯Ӳβ֢Ԥƾһֵָ֬ʹÿʹ֬ʺϸߵĤɷ仯ʹDNARNAṹܵ𺦣𵰰ʷӵĽϸ˥ϣLPOѳΪעĿ⣬ʹŽLPOֵӣд

4.7.ʹ֬ϸ֬ἰϳ֬ôغ֬֯Ʊ֬ϸҺмACTHؿʹ֬ϸֽ֬ᣬͬʱGPs֬28%ҡ֬ϸʾٻ14C-37湲ͬ30ӣⶨ֬ϸÿCPMΪǽϸϳΪָ֬꣬ҺGPsÿ֬ϸõCPMΪδGPs50%ҡʾʹпܳΪµ֬ʴлҩ

5Ѫϵͳã5.1.GPsŨȶ˷ã2.4mg/mlʱǿ4mg/mlʱáþעGPs8mg/kgʹѪѹߣ16mg/kgʱʹѪѹԽͣҶԴҶصµļȱѪTʼS-TƣԶԿáȮעGPs5mg/kg10mg/kgԽȮѪѹѪ״Ѫӹʣʹ-ʱָ½ӷӳļͣļܺѪӰ죬ȵ˲߰ǿעGPs1mg/kgѹѹļܣǿļٶȼӿͬʱӣӶÿʾGPsԴļȱѪٹעбãû뿹йءͣ30ӣٹ20ӣGPs3Oμg/ml50μg/mlŽͲҲʣ50μg/mlʱļøLDHͷšGPsȱѪٹļSODԣԽMDAˮƽļȱѪٹ˾߱áļڽǰ30ӼǻעGPs25mg/kgʹȱѪ24СʱļΧСʹȱѪ6Сʱ10СʱѪἡἤøCPKøLDHԽͣʹȱѪ30ʱȱѪԵļ΢ṹԼᡣ

5.2.⣬GPs50μg/ml100μg/ml200μg/mlܼļϸȱȱˣȱȱ6СʱļϸCPKLDHͷšעGPs10mg/kg25mg/kg30ֹڶʡѪѹѹѹѹ仯ֵָѪѧָԱ仯ļļãǰ30ӸǻעGPs100mg/kgɽ∑STصĵͼSŢܺNSTSTߵĵͼͳѧŲ죬˲߰GS50mg/kgŽãGPs100mg/kgŽ͹Ѫ֬ᣨFFAߣСļΧGS֧ļĴǰǻע100mg/kgGPsFGNG(˲߰)ƹļMDAߣP0.01FGNGʹ󽵵͵SODߣGPsGsҲʹSODߵƣļCPKԽͣGSʹ֮ߣGPsGSҲʹ֮ߣͳѧϽӽԡGPsԼڶݿ˻Կݿ˺Ԥ̷ɢѪѪDICǻעGPs40mg/kgӳ࿨жСʱ䣬ǻעGPs80mg/kgӶ࿨⣬GPs2μg/mlӳķԲӦڣFRP0.01-1μg/mlʱŨԽķԣķӰ졣èעGPs50mg/kgѪѹ½40.7%ά30ϣޱ仯ѹĵð併ѹá

6ѪѪСۼӰ죺6.1.⣬GPs0.25-1.00mg/mlʱԻϩᣨAAյѪСۼдٽѪСãԽԭյѪСۼʹۼߵ¶𽥱СDZӳ˵ɼѪСۼٶȡþעGPs40mg/kgADPAAͽԭյѪСۼãԼ60ӣ5ʱǿ⣬GPsѪСۼŨʱƽԭյѪС5-ɫ5-HTͷţѪСҺcAMPˮƽЧӦءƤעGPs50mg/kgѪСѪ˨Ѫ˨ҪѪС弤£;Ѫ˨ҪѪϵͳ£á

6.2.⣬GPs 0.25-1.00mg/mlѪСѪ˨B2TXB2-6-ͪ-ǰF1α.6-keto-PGF1αɾáIC50ֱΪ1.03mg/ml1.15mgmlGPsAAлѪСۼʵѪ˨γɵĻ֮һ⣬ʹˮ崼ȡ0.25-2gLAAյѪСۼTXB2ͷţTXB2ͷŵIC50Ϊ0.28mg/mlþעȡ35mgkg10Ӻ20ʱAAյѪСۼƣ10-40ڼ䣬ѪСͷTXB2Լ١0.25-4mg/mlͷ6-keto-PGF1αӰ죬ȡԽTXA2ǰлرֵнϺáѪеʹ0.25mg/mlADPյADPغճǵɣյѪС1Ӻ5ۼʣٽ۷Ѫ˨γɾáƶѪСѪϺƶѪӻԣʹѪøʱ䣨KPTTѪøԭʱ䣨PTѪøʱ䣨TTޭ߶ʱ䣨AT߶ʱ䣨RVV-RT߶֬ʱ䣨RVV-CTѪʱӳ⣬мٺϸӾٶȵáҩһ־ѪС幦ܡѪܼϸۼԵȶڵĿѪ˨γҩ߶ܼXѪRVV-CTⶨҪԴ֬ڣRVV-CTⶨѪСѪСӣPE3ĻƷRVV-RTӳԳRVV-CTãӳҩPF3ԡСGPs250mg/kg500mg/kg6죬Իİϸзdzŵá60С߰ϸãСϸӰ졣

7ϵͳãСǻעʹȡ100mg/kg200mg/kgӳͱ˯ʱ䡣СʹࣨGPsԼ20%450mgkgԼСԷʹGPsáС450mg/kgȰ巨֤ʹãСжʱãǿСѹȱãСӾſƣã͸áСGPsl50mg/kg3գӳӾʱ䣬ӳСʱ䣬ǻעGPs200mg/kgӳȱʱ䡣ʱӾѪˮƽͼԭԽͣʹȡ200mgkg14գʹʱӾ󱣳ֽϸѪˮƽټԭģǽʹƣ͵ҪԭGPsɼټע˾С֫ɦٸǻעСŤGPsʹáСƤעʹȡˮ20%Ҵ95%Ҵȡ3.0gkg4-5գɸļϰʹ20%ҴȡԵ׺ϳƼǰùأɵļ乮̲Լ20%Ҵļϰ׿áСǻעѪƽ0.25mg/kg5գʹء¡Է½´кë޹ȣGPs50mg/kg100mg/kg200mg/kg10գԸѪƽءºԷͣͬʱʹк´ë޹ơGPsɸѪƽ൥ʵĺĽãGPsСڵʼִлӰ첻ԣҲԵı䡣 ȱѪٹģ͵ϵأأڶ飬ȱѪǰ15ӸǻעGPsl00mg/kgʹϵԺˮ֯MDAͣGPsԴȱѪٹעԱáùر˫ྱܶͬʱѹ͹ɼԲȫȱѪעGPs50mg/kgԸȱѪ60ӺԵͼ仯ԾѪøLDHἡἤøCPKԣȱѪ̬֯ѧ仯ʾGPsȱѪбá

8ãǻעGPsl0mg/kg10աɷֹٺήԼѪƤʴļ١СGPs70mg/kg350mg/kg6ɷֹήʹߵάC͡ʾGPsڿƤʼظõҩСθʹȡ20գغ衢ҡǰٺʹӹٵʾԺʹԼá ƤעGPs50mg/kg6գȻ̼ѪתøALTߣƬʾϸݱԡԽ󡢻ȾԺתг70%εĴ缰չGPs2Σʹĺ˷Ŀ࣬Ըڶ飬ԸϸдٽáСθʹȡ100mgkg200mg/kg3գģԽѪãԸѪĽ͡ GPsl00mg/kgԤˮɵӦθԱáGPsl00mg/kg5գԴշθáθʮָÿշGPsl00-200mg2-3£Xߺڿ췢ֻߵϻŸơǸ֬շĴ֬Ѫ֢鷢ˣʹѪתøGPs100mg/kg500mg/kgз7ڣʹߵALT½ʾGPsбáСGPs250mg/kg500mg/kg1Σ縹ǻע¸˵Ȼ̼3ղALTGPsʾޱá Сʹ10g/kg30g/kg7գԾֲӦöױĶ֢ãҲƸǻעëϸѪͨ͸ߡ5g/kg7տƽDz˽׹ؽף15g/kg7,׹ؽƵͳѧԡԴѿҲƣ5gkg15g/kgǿȽӽ޼ԡ˵ʹֳ֢֢á Сʹ0.06g/ֻ0.12g/ֻ10գԽȾɫ廥Ϊָ꣬ûͻãʹշͻԽ͡ԻշС΢ˡȾɫ估ӻΪָ꣬GPs֮пձá԰ƽùءơ˿ùCֱΪɳձ TA97TA98TA100TA102ձӣԻر½Ϊָ꣬GPs׿4ձӵձãмЧӦϵ

ԡСʹˮࣨGP10000mg/kg72СʱǻעGPİΪ2862.5mgkgСʹࣨGPsԼ20%İΪ4.5g/kgͬصĽʹ߰߰СǻעֱΪ899.50-1051.32mg/kg1743.25-2049.11mgkgбСǻעGPsİΪ755mg/kgڷ޶ԡǻעʹΪ1850mg/kgڷ10g/kgδԡÿι8g/kg1£һʳѪ򳣹Ͳ֯ѧδ쳣

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