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2017-10-13 21:34 : Ķ:

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ƴM Qián Z

ӢSEMEN STRYCHNI

ԴƷΪǮֲǮStrychnos nuxvomica L. ĸճʵȡӣɹɡ

״Ʒť״ԲΣһ¡һ԰£ֱ1.53cm0.30.6cmܱػɫ״ëмܳʷ״У˿󡣱Ե¡𣬽ϺͻףͻԲ״ꡣʼӲƽɼɫɫ飬״ҶΣҶ57޳ζࡣ

𡿣1Ʒĩһɫëϸʯϸڼ,ϣľϸΣںں֬ͼ

2ȡƷӵ鲿Ƭ1淋Һ1Σ鼴ɫȡƬӷ1ΣԳȺɫ

3ȡƷĩ0.5gȷ-Ҵ10:1Һ5mlŨҺ0.5mlҡ5ӣ2Сʱ˹ҺΪƷҺȡʿǮӼƷȷƳÿ1ml2mgĻҺΪƷҺձɫ׷¼ B飬ȡҺ10μlֱͬһ轺DZϣԼױ-ͪ-Ҵ-ŨҺ4:5:0.6:0.4Ϊչչȡɣϡ⻯ҺƷɫУƷɫӦλϣͬɫİߵ㡣

ոЧҺɫ׷(¼ D)ⶨ

ɫϵͳ ù轺Ϊ-ȼ-״-ŨҺ(42.5:42.5:5:0.35)ΪࣻⲨΪ254nm۰ʿӦ2000

ƷҺƱ ܳȡʿƷӴƳÿ1ml0.4mgҺá

ƷҺƱ ȡƷĩɸԼ0.5gܳƶþ׶ƿУܼȷ25mlŨҺ1mlҡƶ24Сʱٳƶȷ²ʧҡ˹ȡҺ10ml÷Һ©УҺ(3→100)ȡ5Σÿ15mlϲҺŨҺpHֵ910ȷȡ5Σÿ20mlϲȷҺɣӴܽ⣬ת10mlƿ,ϡ̶ȣҡȣá

ⶨ ֱȡƷҺ빩ƷҺ4μlעҺɫǣⶨá

ƷƷ㣬ʿ(C21H22N2O2)ӦΪ1.202.20

ոЧҺɫ׷ ( ¼ D) ⶨ

ɫϵͳ ʮϹ轺Ϊ -10mmol/L 20mmol/L صҺ 10% pH ֵΪ 2.8 21 79 ΪࣻⲨ 260nm ۰ ʿ Ӧ 5000

ƷҺƱ ֱܳȡʿƷ 6mg ǮӼƷ 5mg ֱ 10ml ƿУȷʹܽⲢϡ̶ȣҡȡֱȡ 2ml ͬһ 10 ml ƿУü״ϡ̶ȣҡȣãÿ 1ml кʿ 120 μ g ǮӼ 100 μ g

ƷҺ Ʊ ȡƷĩɸԼ 0.6g ܳƶ þ ׶ƿУҺ 3ml ȣ 30 ӣܼȷ 20ml ƶˮԡϻȡ 2 Сʱ䣬ȷ ʧ ҡȣͨˮƵֽ˹ȥҺ ȡҺ 3ml 10ml ƿУü״ϡ̶ȣҡȣ΢Ĥ 0.45 μ m ˹ ȡҺ á

ⶨ ֱȡƷҺ ƷҺ 10 μ l עҺɫǣⶨá

ƷƷ㣬 ʿ C 21 H 22 N 2 O 2 Ӧ 1.20% ǮӼ C 23 H 26 N 2 O 4 Ӧ 0.90%

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Ǯ ȡǮӣ̷¼ Dɰغɫɫ

ζ࣬£д󶾡

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Ρֹͨʹɢסڷʪԣḻ̌ˣӸʹСԺ֢ʪԹؽʹ

÷0.30.6gƺɢá

ע⡿á÷иá

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ƴM Qián Z

ľʵѶɺ輯ʧ̰ѶĿʵԭʼǰţԭ󷽰ˣҩֲᡷ

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ԴΪǮֲǮʵʱժȥ⣬ȡӣɹɡ

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״ӳʱԲΣť״Ե΢¡𣬳һ԰£һͻֱ13.36ףɫɫɫëףʷ״У˿⣬һͻԲ㣬ԵһСͻԲСͻ֮һߡʼӲ飬ƿʵưɫ͸״ɼεҶ޳ζࡣԾңڳر.ԸʼΪѡ

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ѧɷ֡ľstrychnineǮӼbrucineα-β-³α-, β-colubrineľμvomicineǮ¼novacineicajineľ߰loganinȡ

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βףľһľ֡ͬĥˮ֣֣ܵԼëɨ鷽

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񭴯ĿʵѶۡˮ֣Ƭԡ㡢ݷΪĩĿҶĿնΡɺ輯

οȮǰһĥɷĩˮ̷ʡҽ鷽ط

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ǮʪгɱƬ1.2ǮԼ1824ƬƤϣڻ沿ң󣩣Լ7101ţָΪֹһ֢2μɡ15000˴ΣԼ80Ч

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Ǯˮݣˮˮ1014죬ȥƤеĻڣĻԼ30ӣɫֻ֮ΪȣʱȡڻʯȥʣԼ1014Сʱɸȥʯۣˮϴ1.ɺзۼɷáʼÿ1.5֣3ηÿ3.2ֻ4֡32ýϺõĽЧ1Ч1˹ƣʼЧкãԺ󽥼Ǯƣָӣ޸ӸУ

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ľʵѶʧ̰Ѷʵǰǰӡţ

1.ԡݸĿľĿƻߡ½ʵ¥࣬ľСľɫס

2.ԭʼľľľӴԲ⣬аëζࡣ䶾ľУ䶾ʹϳС֮֫Ρ

ԴҩĻԴΪǮֲǮǮӡ

ֲﶯ1Semen Strychi Nux-vomicae2Semen Strychni Wallichianae

պʹأʵʱժ£ȡӣϴŵĹ⣬ɹɡ

ԭ̬1.ľ10-13mƤɫƤ, ֦⻬ҶҶ5-12mmҶƬʣλԲΣ6-15cm3-9cmȶ˼΢ШλԲΣȫԵ⻬ë3-5ͻϸɲ״ҶҸж̾롣Բ׶״ɡҸ3-5cmֱ2.5-5cmëܰƬСƬСΣȶ˼⣬ëɫ޹ɫȶ5ѣܱëͲ״ȶ5ѣƬΣë5ڻڹܺ˿̣ҩɫԲΣﳤ9.5-12mmԲΣ11mmͷͷ״ڷΡΣֱ2-4cmʱɫʱɫ⻬1-4ţԲΣֱ1-3cmһɫܱɫëڴļ81¡

2.ľʴٱ20mƤҰɫС֦ԲΣ̬ΪɶԵ״ҶҶ5-7mmҶƬʣԲΣ7-12cm3-4.5cmȶ˼̽⣬ШλԲΣȫԵ⻬ëҶΪ3¡Բ׶״ɡԼ4cmܻСëɫȶ5ѣƬΣ汻΢ëԵнëͲ״ưɫԼ10mmȶ5ѣƬΣԼ4mmë5ڻͲĺ˿̣ҩ΢¶ﳤԼ8mmϸͷͷ״ӷΡΣֱ4-6cmʱٺɫӳԲΣ2-3cmƽdzɫë4-6£81¡

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2.ֲϡ

ࡿѧ Ǯϲůʪ򣬲ͺͺѡɽ£΢΢ԣʵɡֳʵɰϻճ֡

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״״1Ǯ ӱԲΣť״ֱ13cm3-6mmԵ΢¡𣬳һ氼£һͻɫɫɫë˿״Եһ¡ߣһСͻףһͻԲ״꣬Լɼһ¡ߡʼӲ顣رԵʿɼɫʷʺ飬벿п϶״εߣҶ2öƱ5-6mm5״߸Լ4mm΢ζ࣬綾

2Ǯ ӳԲΣԵϱ̳״2-3cmֱ15-22cmһɫɫëףܷ״СҶζҶ 3΢ζ࣬綾

Ըʼɫһй߼ѡ

΢ Ӻ棨1Ǯ ƤƤϸһбӳɵϸë500-1000-1700μmԼ25μmںǿľԼ10ߣƿ׼׹ʯϸƤɫǷϵıϸϸΣں񣬰άԣԼɼ˿õҺؽԣϸں֬ͼ2Ǯ Ƥë520-1040μmԼ20μmƽֱŤھ9-10ߣȻɫϸ״ȶ˼ԲԻϣë׻ƿס

1ȡУҺ1gŨ100mlУ1ΣڲӦɫ鷬ľȡһƬŨ1ΣӦԳȺɫǮӼ

2ȡӷĩ08-15gҴ20ml2hʵҡ˹Һȥܼ1molL3mlܽ⣬Һ©У淋pH90ȷ3mlȡ3ΣȷҺŨȡ2-3mlڷҺ©м1molL15mlҡȡֳҺŨἰпСУ󣬼Һ1201ӿӦӣɫ鷬ľ

3ɫ ȡƱҺƷǮӼľΪƷֱڹ轺Gϣ--Ũˮ631չø⻯ԼƷɫУƷɫӦλͬɫߡƷʱ־л񹲺͹ҩ䡷1995涨ƷƷ㺬ľC21H22N2O2ӦΪ120-220

ѧɷ֡ǮӺɷΪͣ“”ϵУnormal series ľstrychnineǮӼbrucine췬ľisostrychnineǮӼisobrucineľN-strychnine NoxideǮӼN-brucine Noxide,β-³֣βcolubrine16-ǻ-β-³֣16hydroxy-β-colubrine16-ǻ-α-³֡“α”ϵУpseudo series αľpseudo-strychnineαǮӼpseudobrucine“N”-׻α”ϵУNmethylpseudo seriesN-׻--αľicajineľμvomicineN-׻--αǮӼno-vacine“”ϵΪ1-3ݡӾ¼ȣ220260棬3min綾ɷַľǮӼԽͣ췬ľǮӼľN-PǮӼN-ﺬߣ3.Ƥľʲ“”ϵΪƤ“α”“N-׻α”ϵΪҶ“N-׻α”ϵΪ[1]ƤľǮӼ4-ǻľ4-hydroxystrychnineľN-ǮӼN-αľαǮӼN-׻--αľľμN-׻--αǮӼβ-³֡⻹ϩѵࣨiridoidǮ߰loganinǮ߰ᣨloganic acidȥǮ߰deoxyloganinǮͪ߰ketologaninǮ߰secologanin[4]

ǮӺɷΪͣ“”ϵ4-ǻ-3--ľ4-hydroxy3methoxystrychnineľǮӼ4-ǻľľN-α-³֡“α”ϵαľαǮӼ“N-׻α”ϵN-׻--αľľμN-׻--αǮӼ4-ǻ-3--N-׻--αľ4hydroxy3methoxy-Nmethyl-secpseudostrychnine[5,6]“”ϵΪ[5]Ƥľʲ“”ϵΪƤ“α”“N-׻α”ϵΪҶ“N-׻α”ϵΪ[5]⣬ҶN---αľN-cyano-secpseudostrychnineN---αǮӼNcyano-sec-pseudobrucine[78]

ҩá1.ϵͳ Ʒķľϵͳ˷ã˷ܼķܣ˷ĺ༰Ѫ˶࣬ߴƤʵĸоܡ

1.1.Լ Էľи߶ȵԣƤע0.02%ᷬľҺ0.5mlɼ˷ߣᴥƤ棬سԵèע0.01%ᷬľ0.2mg/kgߡʱɳǿֱԾʡƼķľʹ񾭳嶯ڼдף߼跴˷ԣ˿̼跴ʱ䣬߷ǿȣҲƻĽƹ̣жķľƻ˽ƹ̡ǿֱԾʡľԹУƵøķֽ⣬Ϊ˼Ϊľ˷òԭΪϵڷľͻԽʸʰᾺͻƲλѡ˶ԪмԪͻƣʹ˶ԪԴ̼ϴӦͬʱҲϼϸ(RenshawCell)˶Ԫã˶Կ(켡)֮Ľƣ˴ǿֱԾʡ

1.2. ϼأԵȮע2mg/kgᷬľѪѹ(10-20mmHg)ʱڸ߶ˮƽƻ裬Ѫѹ漴½ľѪѹҲl˵ľѪ˶˷ԣѪҺѭľ˷ãɳĶ컺ľߺ˷ԣʹӿ죬ر౻ʱЩøԡ⣬˷ܿࡣ

1.3.ԴƤʵ СķľܼǿƤʵ˷ܹ̣ʹ״̬IJѡζӾȸоٵĹܡƼķľȮͺʵ񾭹֢ãܴʹ˷ܺƹ֮ϵĻָķľ(ӽж)ڶݵ˷ܹ̺󣬼

2.ܰϸ ٴбᷬľϰƶѪЧȹ۲ǮӷּҺͬܰϸ˿ָӰ죬ҺŨΪ0.3-0.7mg/mlʱָһ£ŨΪ0.2mg/mlʱָһ£ŨΪ0.1mg/mlʱָ9.975%l顣

3.ϵͳ ǿҿζɴ̼ζθҺڣٽܺʳ˳֤ľθƽ˷á

4.ԺϵͳӰ ½άҩҩЭ鱨Զȷ֤ǮӼ50mg/kgθСԵãǿȳɴСӺڷ֤ǮӼ40mg/kgԵ̵ãǿȻŲƽʵ鷢֣ҩʱӳʱܼǿÿ֯á

5.ãǮӵˮ(12)ԹڶϻѢ¶ŰСѿѢв̶ͬȵá0.1%ǮӼȫѪ˾˫Ϳ

6. SollmanTAǮӼԸоĩã5-10%ǮӼҺʹǻճĤǮӼͷľʱ񾭼⴫ּã嶯ϱȫԾڸǣֻ񾭼걾ϲֳܱ

ԡж߳нУѣȫȻ켡ͬʱǿֱԾʡðͱҩᄇעƾʣøϴθԣСƤעİʿΪ0.47mg/kg0.85mg/kg4-ǻʿΪ0.556mg/kgǮӼעΪ8mg/kg

ľǮӼǮʶСθļ԰ֱΪ3.27233234.5mg/kgСǻעļ԰ֱΪ1.536977.76mg/kgǮӼȮעİΪ8mg/kg1η5-10mgķľж30mgǮư׺ܼ50.54mgʱжзǮ7жIJ档ԭΪǿֱԾʷ˥߼Ϣ1988˱ȽǮӼͬƷĶԡ

ơǮӷۣȡɳӣùڳȣ𾻵ǮӣɫȡɸȥɰӣȥëзۡǮӣȡ𾻵ǮӣˮУȡˮݣ̳ȥƤë΢гɱƬȡùȣǮƬ΢ɫȡĿԶƹ֮

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Ρͨ磻ǿɢ᣻ֹʹףⶾʪʹḻ̌֫ˣʹӸҴԣʹݷ磻Ѣ

÷ڷƺ衢ɢÿ0.2-0.6g0.9gãĩˮĥͿ򰾸̯ڷ簴ɷַʿ㣬һ6mgΪˡڷһСʼ𽥼оһ΢ΪȡЧ˷ӦҲټ

ע⡿и߼ɷݾ衷ѪƢθʵߣ֮ǮӺзľ5-10mgɷж30mgǮư׺ܼ50.54mgʱжзת(ҪɷΪǮ)жǮ7жIJ

1.Ŀ˺ȲʺʹƦ飬֭֮ĥˮʡ

2.ҩ־ɢѪȣ׶Ӹң񴯡

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ƴM Qián Z

ԴΪǮǮֲǮStrychnos wallichiana Steud. ex DC.[S. pierriana auct. non A. W. Will]ǮS. nux-vomica L.Ըҩ

ԭ̬1Ǯ ľʴٱƤҰɫС֦Բ״⻬ҸɶԣҶҶ57棻ҶƬʣԲΣ712ף34.5ףȶ˶̽⣬ШλԲΣȫԵ⻬ë֦ҿɫ״5ѣڹԲͲ״ϲ5ѣ5ڻڹܺ˿̣ҩšΣʱٺɫֱ46סӳԲζ⣬ܱdzɫë

ȴʯҵľСҹʡзֲ

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